Mesothelioma antibody

Antigen: Mesothelioma

Clonality: Monoclonal (HBME-1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN104136

Additional Information

Immunogen: BALB/C mice were injected with suspension of human mesothelioma cells from patients with malignant epithelial mesothelioma.

Format: Ig-fraction

Isotype: IgM  (Matching secondary antibodies)

Clone: HBME-1

Specificity: This antibody reacts with an unknown antigen on microvilli of mesothelioma cells. It stains normal mesothelial cells as well as epithelial mesotheliomas in a thick membrane pattern due to abundant lung microvilli on the surface of these cells.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:15-1:25 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Mesothelioma. Cellular localization: Cell membrane

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cancer

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human mesothelioma stained with Mesothelioma antibody

Publications

Publications: Fetsch, Abati, Hijazi: "Utility of the antibodies CA 19-9, HBME-1, and thrombomodulin in the diagnosis of malignant mesothelioma and adenocarcinoma in cytology." in: Cancer, Vol. 84, Issue 2, pp. 101-8, 1998 (PubMed).

Dahlstrom, Maxwell, Brodie et al.: "Distinctive microvillous brush border staining with HBME-1 distinguishes pleural mesotheliomas from pulmonary adenocarcinomas." in: Pathology, Vol. 33, Issue 3, pp. 287-91, 2001 (PubMed).

Fetsch, Simsir, Abati: "Comparison of antibodies to HBME-1 and calretinin for the detection of mesothelial cells in effusion cytology." in: Diagnostic cytopathology, Vol. 25, Issue 3, pp. 158-61, 2001 (PubMed).