MIC2 Gene Protein antibody

Antigen: MIC2 Gene Protein

Clonality: Monoclonal (12E7)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN104203

Additional Information

Immunogen: BALB/C mice were injected with acute lymphocytic leukemia T-cells.

Format: Supernatant (prediluted)

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: 12E7

Specificity: This antibody reacts with the MIC2 gene products expressed on the cell membrane of lymphocytes, cortical thymocytes, Langerhans' islet cells, granulosa cells of the ovary and Sertoli's cells of the testis. This antibody reacts strongly with Ewing's Sarcoma and peripheral neuroectodermal tumors.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Tonsil. Cellular localization: Cell membrane

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: in:

Fellinger, Garin-Chesa, Glasser et al.: "Comparison of cell surface antigen HBA71 (p30/32MIC2), neuron-specific enolase, and vimentin in the immunohistochemical analysis of Ewing's sarcoma of bone." in: The American journal of surgical pathology, Vol. 16, Issue 8, pp. 746-55, 1992 (PubMed).

Ambros, Ambros, Strehl et al.: "MIC2 is a specific marker for Ewing's sarcoma and peripheral primitive neuroectodermal tumors. Evidence for a common histogenesis of Ewing's sarcoma and peripheral primitive neuroectodermal tumors from MIC2 expression and specific chromosome aberration." in: Cancer, Vol. 67, Issue 7, pp. 1886-93, 1991 (PubMed).