Annexin A5 (ANXA5) antibody

Details for Product No. ABIN1042258
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Antigen
Synonyms anx, anx5, ANX V, anxa5, cb989, wu:fa98f06, wu:fj10f10, MGC89158, ANX5, ENX2, PP4, RPRGL3, Anx5, R74653, LC5, enx2
Reactivity
Human, Zebrafish
(125), (45), (43), (21), (14), (12), (12), (4), (3), (1), (1), (1)
Host
Mouse
(109), (34)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(14), (12), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(106), (62), (35), (34), (21), (20), (20), (16), (16), (14), (3), (2), (1)
Pubmed 1 reference available
Quantity 1 mL
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Catalog No. ABIN1042258
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Clone RUU-WAC2A
Isotype IgG1
Specificity RUU-WAC2A reacts against human recombinant and endogenous annexin A5. It shows no cross reactivity with Annexins I, II, III, IV, VI, VII and VIII in ELISA or in immunoprecipitation. It does not recognise monkey and rat Annexin V in ELISA.
Characteristics Annexins
Purification Supernatant
Alternative Name annexin V
Background Annexin A5 (or annexin V) is a cellular protein of the annexin family. Annexin A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin, and also to inhibit the activity of phospholipase A1. Annexin A5 forms a shield around negatively-charged phospholipid molecules, which blocks the entry of phospholipids into coagulation (clotting) reactions. Antibodies directed against annexin A5 are the cause of the antiphospholipid syndrom in which the formation of the shield is disrupted by antibodies. Without the shield, there is an increased quantity of phospholipid molecules on cell membranes, speeding up coagulation reactions and causing the blood-clotting characteristic of the antiphospholipid antibody syndrome. Annexin A5 is used as a probe in the annexin A5 affinity assay to detect cells that have expressed phosphatidylserine on the cell surface, a feature found in apoptosis aswell as other forms of cell death (1,2). Platelets also expose phosphatidyl-serine on their surface when activated, which serves as binding site for various coagulation factors. Annexin A5 has been shown to interact with kinase insert domain receptor (3) and integrin beta 5 (4). RUU-WAC2a is a monoclonal that specifically reacts with annexin A5. This monoclonal antibody is directed to the phospholipid binding domain of annexin A5 and as such it inhibits annexin A5 binding to anionic phospholipid membranes for more than 95%. RUU-WAC 2A does not remove annexin A5 bound to phospholipid surfaces indicating that the epitope is not accessible after interaction with annexin A5.
Research Area Apoptosis/Necrosis
Application Notes The monoclonal antibody is suitable for ELISA in combination with a polyclonal antibody raised against Annexin V in rabbit. It can be applied in immunofluorescence to parafolmaldehyde fixed cell samples and in immunohistochemistry of frozen sections and formaldehyde fixed and paraffin embedded tissue specimens. For immunohistochemistry of paraffin sections a pretreatment step with 2% amino-propyl-triethoxysilan (in 100% ethanol) and 0,2 % BSA is recommended. The sections are heated for 15 minutes at 100°C in a citrate buffer (10 mM trisodium citrate, pH 6,0). The sections are then further processed by routine procedures (see below). RUU-WAC2a inhibits annexin A5 binding to anionic phospholipid-containing vesicles, activated platelets and apoptotic cells for more than 95%. Optimal antibody dilution should be determined by titration, recommended range is 1:2 – 1:5 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:5 – 1:25 for immunoblotting applications.
Comment

RUU-WAC2a is a mouse monoclonal IgG1 antibody obtained by fusion of P3-X63-Ag 8,653 mouse myeloma cells with spleen cells from a BABL/c mouse immunized with recombinant annexin A5.

Restrictions For Research Use only
Format Liquid
Buffer 0.09% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Product cited in: Koopman, Reutelingsperger, Kuijten et al.: "Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis." in: Blood, Vol. 84, Issue 5, pp. 1415-20, 1994 (PubMed).

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