Membrane Metallo-Endopeptidase (MME) antibody

Antigen: Membrane Metallo-Endopeptidase (MME)

Synonyms: NEP, CD10, CALLA, MGC126681, MGC126707, DKFZp686O16152, C85356, 6030454K05Rik, MME

Clonality: Monoclonal (B-E3)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN1042265

Additional Information

Alternative name: CD10

Isotype: IgG2a

Clone: B-E3

Description: CD10 (Cluster of Differentiation 10), also known as neprilysin, membrane metallo-endopeptidase, neutral endopeptidase (NEP), and common acute lymphoblastic leukemia antigen (CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably the amyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. Synthesized as a membrane-bound protein, the neprilysin ectodomain is released into the extracellular domain after it has been transported from the Golgi apparatus to the cell surface. In neurons, neprilysin is regulated by the protein nicastrin, a component of the gamma secretase complex that performs a necessary step in processing amyloid precursor protein to amyloid beta. CD10 is thought to down regulate cellular responses to peptide hormones by degrading the peptide bonds and reducing the concentration of peptide available for receptor binding and signal transduction. It does not inhibit the mixed lymphocyte culture reaction and CD10 antibodies may exert a cytotoxic effect on CD10+ cells in association with rabbit complement. CD10 on neutrophils limits their inflammatory responses and CD10 expression on bone marrow stromal cells appears to regulate B cell development. CD10 is expressed on early B and T lymphoid precursors, B blasts, some granulocytes and bone marrow stromal cells. CD10 is also expressed on various epithelia, some smooth muscle and myoepithelial cells, brain and fibroblasts. CALLA is also present on other types of B and T cell tumors and on normal peripheral blood granulocytes.

Characteristics: CD markers

Specificity: B-E3 recognizes a 100 kD membrane antigen and is specific for the human common acute lymphoblastic leukemia antigen (CALLA).

Comments:

B-E3 is a mouse monoclonal IgG2a antibody raised against CD10.

Application Details

Application Notes: B-E3 can be applied in flow cytometry for analysis of blood and bone marrow samples or in immunohistochemistry (IHC) using cytospots or frozen tissue sections. Optimal antibody dilution should be determined by titration. B-E3 is widely used as a marker of common (pre-B) acute lymphocytic leukemias and certain lymphomas in flow cytometry and IHC. It is also used in the diagnosis of a subset of follicular lymphomas.

Purification: Purified

Buffer: 0.01 M sodium phosphate, 0.15 M NaCl, 0.2% BSA, 0.09% sodium azide, pH 7.3.

Storage: Store at 4°C.

Research Area: Cancer, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Adaptive Immunity, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Product: Shipp, Look: "Hematopoietic differentiation antigens that are membrane-associated enzymes: cutting is the key!" in: Blood, Vol. 82, Issue 4, pp. 1052-70, 1993 (PubMed).