CD163 Molecule (CD163) antibody

Antigen: CD163 Molecule (CD163)

Synonyms: CD163, DMBT1, M130, MM130, CD163v2, CD163v3

Clonality: Monoclonal (MAC2-48)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Flow Cytometry (FACS), Western Blotting (WB)

Catalog no.: ABIN1042308

Additional Information

Alternative name: CD163

Isotype: IgG1

Clone: MAC2-48

Description: CD163 (Cluster of Differentiation 163) is a human protein encoded by the CD163 gene.] It has also been shown to mark cells of monocyte/macrophage lineage. The CD163 antigen is the cysteine-rich human scavenger receptor protein (p155, M130) found on mononuclear phagocytes, monocytes, and macrophages. CD163 is up-regulated on mononuclear phagocytes by IL-4, IL-6 and dexamethasone and serves as a receptor for the hemoglobin / haptoglobin complex. Expression of CD163 is restricted to cells of monocyte lineage and increases as the monocytes mature into macrophages. CD163 is present on all CD14 + monocytes, most CD64+ monocytes, and demonstrates higher expression on CD16+ monocytes. Stimulation of monocytes and macrophages by lipopolysacharide (LPS), IL-10, and tumor necrosis factor alpha (TNF-alpha) causes shedding of CD163 from the cell surface into plasma or supernatant.

Characteristics: CD markers

Specificity: The CD163 antigen is the cysteine-rich human scavenger receptor protein (p155, M130) found on mononuclear phagocytes, monocytes, and macrophages. CD163 is up-regulated on mononuclear phagocytes by IL-4, IL-6 and dexamethasone and serves as a receptor for the hemoglobin / haptoglobin complex. Expression of CD163 is restricted to cells of monocyte lineage and increases as the monocytes mature into macrophages. CD163 is present on all CD14 + monocytes, most CD64+ monocytes, and demonstrates higher expression on CD16+ monocytes. Stimulation of monocytes and macrophages by lipopolysacharide (LPS), IL-10, and tumor necrosis factor alpha (TNF-alpha) causes shedding of CD163 from the cell surface into plasma or supernatant.

Comments:

MAC2-48 is a mouse monoclonal IgG1 antibody raised against CD163.

Application Details

Application Notes: CD163, Clone MAC2-48 can be applied in flow cytometry, immunohistochemistry using paraffin embedded tissue sections, western blots and ELISA. Optimal antibody dilution should be determined by titration.

Purification: Purified

Buffer: 0.01 M sodium phosphate, 0.15 M NaCl, 0.2% BSA, 0.09% sodium azide, pH 7.3.

Storage: Store at 4°C.

Research Area: Cardiovascular, Atherosclerosis, CD Antigens, Innate Immunity, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Product: Morganelli, Guyre: "IFN-gamma plus glucocorticoids stimulate the expression of a newly identified human mononuclear phagocyte-specific antigen." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 7, pp. 2296-304, 1988 (PubMed).

Buechler, Ritter, Orsó et al.: "Regulation of scavenger receptor CD163 expression in human monocytes and macrophages by pro- and antiinflammatory stimuli." in: Journal of leukocyte biology, Vol. 67, Issue 1, pp. 97-103, 2000 (PubMed).

Sulahian, Högger, Wahner et al.: "Human monocytes express CD163, which is upregulated by IL-10 and identical to p155." in: Cytokine, Vol. 12, Issue 9, pp. 1312-21, 2000 (PubMed).

Madsen, Møller, Nielsen et al.: "Molecular characterization of the haptoglobin.hemoglobin receptor CD163. Ligand binding properties of the scavenger receptor cysteine-rich domain region." in: The Journal of biological chemistry, Vol. 279, Issue 49, pp. 51561-7, 2004 (PubMed).

Moestrup, Møller: "CD163: a regulated hemoglobin scavenger receptor with a role in the anti-inflammatory response." in: Annals of medicine, Vol. 36, Issue 5, pp. 347-54, 2004 (PubMed).

Lau, Chu, Weiss: "CD163: a specific marker of macrophages in paraffin-embedded tissue samples." in: American journal of clinical pathology, Vol. 122, Issue 5, pp. 794-801, 2004 (PubMed).

Davis, Zarev: "Human monocyte CD163 expression inversely correlates with soluble CD163 plasma levels." in: Cytometry. Part B, Clinical cytometry, Vol. 63, Issue 1, pp. 16-22, 2005 (PubMed).