Glycophorin A (MNS Blood Group) (GYPA) antibody

Antigen: Glycophorin A (MNS Blood Group) (GYPA)

Synonyms: MN, GPA, MNS, GPSAT, CD235a, GPErik, HGpMiV, HGpMiX, GpMiIII, HGpMiXI, HGpMiIII, HGpSta(C), AI853584, GYPA, gpa

Clonality: Monoclonal (NaM10-6G4)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN1042352

Additional Information

Alternative name: CD235a

Isotype: IgG2a

Clone: NaM10-6G4

Description: Glycophorin A (MNS blood group), also known as GYPA, is a protein which in humans is encoded by the GYPA gene. GYPA has also recently been designated CD235a (cluster of differentiation 235a). CD235a is not expressed on lymphoid or granulocytic progenitor cells and therefore is very useful as a marker for detection of the erythroid cell lineage. CD235a seems to be absent from erythroid colony forming cells in bone marrow, but is present during maturation from (pro- )erythroid blast cells to mature erythrocytes. CD235a is clinically important in the classification of leukemias. Using CD235a antibodies, a distinction can be made between erythroleukemia and other (acute) leukemias like e.g. myeloid, lymphoid or undifferentiated leukemias. CD235a is often used in combination with detection of H-antigen and/or anti-CD36 antibodies for additional characterization of early erythroid cells. Additional immunophenotyping of early erythroleukemias can be performed by detecting myeloid-associated antigens such as CD13, CD14, CD15, CD33 and CD34, to discriminate from lymphoid lineage-associated antigens like CD2, CD7, CD10 and CD19. Glycophorin A (GpA) antigen (CD235a) is detected by the monoclonal antibody NAM10-6G4 and is exclusively expressed on human erythroid cells and their progenitors. These antibodies may be effectively used for differentiation in acute leukemias e.g. presence of the GpA antigen on leukemia cells indicates an early erythroid cell lineage of the tumor cells.

Characteristics: CD markers

Specificity: Clone NAM10-6G4 produces mouse IgG2a immunoglobulins directed against the human CD235a antigen (41 kD) which is expressed as a cell surface sialoglycoprotein.

Comments:

NaM10-6G4 is a mouse monoclonal IgG2a antibody raised against CD235a.

Application Details

Application Notes: CD235a antibodies, clone NAM10-6G4, can be applied in flow cytometry for analysis of blood and bone marrow samples or in immunohistochemistry using frozen tissue sections. CD235a antibodies are used to identify erythroid cells during hematopoietic differentiation. Optimal antibody dilution should be determined by titration.

Purification: Purified

Buffer: 0.01 M sodium phosphate, 0.15 M NaCl, 0.2% BSA, 0.09% sodium azide, pH 7.3.

Storage: Store at 4°C.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Product: Cuneo, Van Orshoven, Michaux et al.: "Morphologic, immunologic and cytogenetic studies in erythroleukaemia: evidence for multilineage involvement and identification of two distinct cytogenetic-clinicopathological types." in: British journal of haematology, Vol. 75, Issue 3, pp. 346-54, 1990 (PubMed).

Villeval, Cramer, Lemoine et al.: "Phenotype of early erythroblastic leukemias." in: Blood, Vol. 68, Issue 5, pp. 1167-74, 1986 (PubMed).

Loken, Shah, Dattilio et al.: "Flow cytometric analysis of human bone marrow: I. Normal erythroid development." in: Blood, Vol. 69, Issue 1, pp. 255-63, 1987 (PubMed).

Lang: "The roentgenographic assessment of bladder tumors. A comparison of the diagnostic accuracy of roentgenographic techniques." in: Cancer, Vol. 23, Issue 3, pp. 717-24 (PubMed).

Liszka, Majdic, Bettelheim et al.: "Glycophorin A expression in malignant hematopoiesis." in: American journal of hematology, Vol. 15, Issue 3, pp. 219-26, 1983 (PubMed).

DIDISHEIM, TROMBOLD, VANDERVOORT et al.: "ACUTE PROMYELOCYTIC LEUKEMIA WITH FIBRINOGEN AND FACTOR V DEFICIENCIES." in: Blood, Vol. 23, pp. 717-28 (PubMed).