MutS Homolog 2 (E. Coli) (MSH2) antibody

Antigen: MutS Homolog 2 (E. Coli) (MSH2)

Clonality: Monoclonal (25D12)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN104246

Additional Information

Alternative name: Mismatch Repair Protein (MSH2)

Immunogen: BALB/C mice were injected with recombinant human MSH2 protein.

Format: Supernatant

Isotype: IgG1 kappa

Clone: 25D12

Specificity: This antibody reacts with a 102 kD protein known as MSH2 (Mismatch Repair protein 2). MSH2 protein is involved in the initial recognition of mismatched nucleotides during the post replication mismatch repair process. Loss of MSH2 function leads to the accumulation of replication errors, which in turn may be responsible for the multiple mutations required for multistage carcinogenesis.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Purification: Supernatant

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Kolodner, Hall, Lipford et al.: "Structure of the human MSH2 locus and analysis of two Muir-Torre kindreds for msh2 mutations." in: Genomics, Vol. 24, Issue 3, pp. 516-26, 1995 (PubMed).

Fishel, Lescoe, Rao et al.: "The human mutator gene homolog MSH2 and its association with hereditary nonpolyposis colon cancer." in: Cell, Vol. 75, Issue 5, pp. 1027-38, 1994 (PubMed).

Maliaka, Chudina, Belev et al.: "CpG dinucleotides in the hMSH2 and hMLH1 genes are hotspots for HNPCC mutations." in: Human genetics, Vol. 97, Issue 2, pp. 251-5, 1996 (PubMed).

Palombo, Iaccarino, Nakajima et al.: "hMutSbeta, a heterodimer of hMSH2 and hMSH3, binds to insertion/deletion loops in DNA." in: Current biology : CB, Vol. 6, Issue 9, pp. 1181-4, 1997 (PubMed).

Bressenot, Marcon, Montagne et al.: "Clear cell adenocarcinoma: a rare variant of primary colonic tumour." in: International journal of colorectal disease, Vol. 23, Issue 1, pp. 137-8, 2007 (PubMed).