Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) antibody

Antigen: Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC)

Synonyms: MUC5, MUC5AC, LOC100188911

Clonality: Monoclonal (45M1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN104325

Additional Information

Alternative name: Mucin 5AC

Immunogen: BALB/C mice were injected with M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le (a-b) patient.

Format: Ig-fraction

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: 45M1

Specificity: This antibody reacts with a 1000 kD protein and recognizes the peptide core of gastric mucin M1. Mucins are high molecular weight glycoproteins with 80% carbohydrates and 20% core protein. Gastric mucin M1 antigen is found in columnar mucus cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon but not in normal colon. Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric mucin in the adult colon or due to changes in the glycosylation pattern of mucin, which expose the hidden M1 antigen. It cross-reacts with monkey, rabbit, cat, mouse, rat, pig, hedgehog, and chicken.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. This anti body is suitable for Formalin fixed paraffin embedded tissue section and does not require any pretreatment. Positive Control: Stomach. Cellular localization: Cytoplasmic, cell surface

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Extracellular Matrix

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded stomach stained with Mucin 5AC antibody

Publications

Publications: Bara, Decaens, Loridon-Rosa et al.: "Immunohistological characterization of mucin epitopes by pre-treatment of gastro-intestinal sections with periodic acid." in: Journal of immunological methods, Vol. 149, Issue 1, pp. 105-13, 1992 (PubMed).

Bara, Gautier, Mouradian et al.: "Oncofetal mucin M1 epitope family: characterization and expression during colonic carcinogenesis." in: International journal of cancer. Journal international du cancer, Vol. 47, Issue 2, pp. 304-10, 1991 (PubMed).