p65 antibody (Nuclear Factor-KB P65) (C-Term)

Details for Product anti-NFkBP65 Antibody No. ABIN1043700, Supplier: Log in to see
Antigen
  • NFKB3
  • p65
  • NFkB
  • v-rel avian reticuloendotheliosis viral oncogene homolog A
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • RELA
  • Rela
Alternatives
anti-Human p65 antibody for Immunofluorescence (fixed cells)
Epitope
C-Term
136
72
66
58
51
47
43
40
39
36
33
32
25
21
18
17
16
15
15
13
13
13
12
11
10
9
9
8
7
6
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1207
676
518
73
59
55
31
30
28
25
15
8
8
6
5
4
2
1
1
Host
Rabbit
1036
147
17
16
11
1
Clonality
Polyclonal
Conjugate
This p65 antibody is un-conjugated
34
34
26
21
19
18
16
16
13
13
13
9
9
9
9
9
9
8
8
4
4
3
3
3
2
2
2
2
2
2
1
Application
Gel Shift (GS), Western Blotting (WB)
880
429
357
269
224
149
118
88
84
28
22
18
18
11
11
6
6
6
5
4
4
2
2
1
Options
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Immunogen NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Peptide
Specificity This product was prepared from monospecific antiserum by delipidation and defibrination. Anti-NFkB p65 (Rel A) may react non-specifically with other proteins. Control peptide (code #100-4165p) will compete only with the specific reaction of antiserum with the NFkB p65 (Rel A) subunit.
Characteristics Anti NFkB p65 Antibody recognizes NFKB p65 which is a component of NFKB. NFKB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkappaB bound to IkappaB. NFkappaB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkappaB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkappaBalpha. Ideal for Cardiovascular, Cell Biology, Immunology, Signal Transduction research.
Purification Ascites
Sterility Sterile filtered
Alternative Name NFKB p65 (NFkBP65 Antibody Abstract)
Background Anti NFkB p65 Antibody recognizes NFKB p65 which is a component of NFKB. NFKB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkappaB bound to IkappaB. NFkappaB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkappaB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkappaBalpha. Ideal for Cardiovascular, Cell Biology, Immunology, Signal Transduction research.
Synonyms: NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB, Nuclear factor NF-kappa-B p65 subunit
Gene ID 5970, 223468676
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades
Application Notes Anti-NFkB p65 (Rel A) is suitable for the detection by immunoblot of human and mouse NFkB p65 (Rel A). This product was also tested in a gel supershift assay and found to be reactive against all p65 (Rel A) containing human, mouse or rat NFkB complexes using 0.5 to 1.0 µl per assay.
Comment

Gene Name: RELA

Restrictions For Research Use only
Format Liquid
Concentration 80 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months
Supplier Images
 image for anti-p65 antibody (Nuclear Factor-KB P65) (C-Term) (ABIN1043700) anti-Nuclear Factor-KB P65 (NFkBP65) (C-Term) antibody
 image for anti-p65 antibody (Nuclear Factor-KB P65) (C-Term) (ABIN1043700) anti-Nuclear Factor-KB P65 (NFkBP65) (C-Term) antibody (Image 2)
Product cited in: Ghosh, Yang, Rothstein, Robinson: "Nuclear factor-κB contributes to neuron-dependent induction of glutamate transporter-1 expression in astrocytes." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 25, pp. 9159-69, 2011 (PubMed).

Yoon, Korade, Carter: "Protein kinase A-induced phosphorylation of the p65 subunit of nuclear factor-kappaB promotes Schwann cell differentiation into a myelinating phenotype." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 14, pp. 3738-46, 2008 (PubMed).

Begley, Kasina, Mehra, Adsule, Admon, Lonigro, Chinnaiyan, Macoska: "CXCL5 promotes prostate cancer progression." in: Neoplasia (New York, N.Y.), Vol. 10, Issue 3, pp. 244-54, 2008 (PubMed).

Lou, Kaplowitz: "Glutathione depletion down-regulates tumor necrosis factor alpha-induced NF-kappaB activity via IkappaB kinase-dependent and -independent mechanisms." in: The Journal of biological chemistry, Vol. 282, Issue 40, pp. 29470-81, 2007 (PubMed).

Cao, Zhang, Edwards, Mosser: "NF-kappaB1 (p50) homodimers differentially regulate pro- and anti-inflammatory cytokines in macrophages." in: The Journal of biological chemistry, Vol. 281, Issue 36, pp. 26041-50, 2006 (PubMed).

Poser, Golob, Buettner, Bosserhoff: "Upregulation of HMG1 leads to melanoma inhibitory activity expression in malignant melanoma cells and contributes to their malignancy phenotype." in: Molecular and cellular biology, Vol. 23, Issue 8, pp. 2991-8, 2003 (PubMed).

Jobin, Bradham, Russo, Juma, Narula, Brenner, Sartor: "Curcumin blocks cytokine-mediated NF-kappa B activation and proinflammatory gene expression by inhibiting inhibitory factor I-kappa B kinase activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 6, pp. 3474-83, 1999 (PubMed).

Unlap, Jope: "Dexamethasone attenuates NF-kappa B DNA binding activity without inducing I kappa B levels in rat brain in vivo." in: Brain research. Molecular brain research, Vol. 45, Issue 1, pp. 83-9, 1997 (PubMed).

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