Nuclear Factor-KB P65 (NFkBP65) (C-Term) antibody

Details for Product No. ABIN1043700
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Antigen
Synonyms c-Rel, C-Rel, Xrel2, c-rel, rel-A, v-rel, xrel, NFKB3, p65, NFkB, zgc:100833
Epitope
C-Term
(66), (56), (46), (46), (36), (34), (33), (31), (31), (30), (28), (20), (19), (12), (11), (7), (7), (7), (7), (6), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(852), (442), (351), (76), (75), (74), (38), (9), (8), (6), (2), (2), (1), (1)
Host
Rabbit
(799), (65), (6), (5), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(24), (19), (15), (11), (10), (10), (10), (10), (10), (10), (10), (10), (10), (10), (1), (1), (1)
Application
Western Blotting (WB), Gel Shift (GS)
(653), (335), (332), (170), (128), (121), (100), (38), (20), (18), (16), (12), (7), (5), (2), (1)
Pubmed 8 references available
Quantity 25 µL
Options
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Catalog No. ABIN1043700
126.50 $
Plus shipping costs $45.00 and if applicable $26.40 dry ice

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Immunogen NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Peptide
Specificity This product was prepared from monospecific antiserum by delipidation and defibrination. Anti-NFkB p65 (Rel A) may react non-specifically with other proteins. Control peptide (code #100-4165p) will compete only with the specific reaction of antiserum with the NFkB p65 (Rel A) subunit.
Characteristics Anti NFkB p65 Antibody recognizes NFKB p65 which is a component of NFKB. NFKB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkappaB bound to IkappaB. NFkappaB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkappaB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkappaBalpha. Ideal for Cardiovascular, Cell Biology, Immunology, Signal Transduction research.
Purification Ascites
Sterility Sterile filtered
Alternative Name NFKB p65
Background Anti NFkB p65 Antibody recognizes NFKB p65 which is a component of NFKB. NFKB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkappaB bound to IkappaB. NFkappaB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkappaB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkappaBalpha. Ideal for Cardiovascular, Cell Biology, Immunology, Signal Transduction research.
Synonyms: NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB, Nuclear factor NF-kappa-B p65 subunit
Gene ID 5970, 223468676
Application Notes Anti-NFkB p65 (Rel A) is suitable for the detection by immunoblot of human and mouse NFkB p65 (Rel A). This product was also tested in a gel supershift assay and found to be reactive against all p65 (Rel A) containing human, mouse or rat NFkB complexes using 0.5 to 1.0 µl per assay.
Comment

Gene Name: RELA

Restrictions For Research Use only
Format Liquid
Concentration 80 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  Expiration date is one (1) year from date of opening.
Expiry Date 12 months
Supplier Images
anti-Nuclear Factor-KB P65 (NFkBP65) (C-Term) antibody anti-Nuclear Factor-KB P65 (NFkBP65) (C-Term) antibody
anti-Nuclear Factor-KB P65 (NFkBP65) (C-Term) antibody (2) anti-Nuclear Factor-KB P65 (NFkBP65) (C-Term) antibody (Image 2)
Product cited in: Unlap, Jope: "Dexamethasone attenuates NF-kappa B DNA binding activity without inducing I kappa B levels in rat brain in vivo." in: Brain research. Molecular brain research, Vol. 45, Issue 1, pp. 83-9, 1997 (PubMed).

Jobin, Bradham, Russo et al.: "Curcumin blocks cytokine-mediated NF-kappa B activation and proinflammatory gene expression by inhibiting inhibitory factor I-kappa B kinase activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 6, pp. 3474-83, 1999 (PubMed).

Poser, Golob, Buettner et al.: "Upregulation of HMG1 leads to melanoma inhibitory activity expression in malignant melanoma cells and contributes to their malignancy phenotype." in: Molecular and cellular biology, Vol. 23, Issue 8, pp. 2991-8, 2003 (PubMed).

Cao, Zhang, Edwards et al.: "NF-kappaB1 (p50) homodimers differentially regulate pro- and anti-inflammatory cytokines in macrophages." in: The Journal of biological chemistry, Vol. 281, Issue 36, pp. 26041-50, 2006 (PubMed).

Lou, Kaplowitz: "Glutathione depletion down-regulates tumor necrosis factor alpha-induced NF-kappaB activity via IkappaB kinase-dependent and -independent mechanisms." in: The Journal of biological chemistry, Vol. 282, Issue 40, pp. 29470-81, 2007 (PubMed).

Begley, Kasina, Mehra et al.: "CXCL5 promotes prostate cancer progression." in: Neoplasia (New York, N.Y.), Vol. 10, Issue 3, pp. 244-54, 2008 (PubMed).

Yoon, Korade, Carter: "Protein kinase A-induced phosphorylation of the p65 subunit of nuclear factor-kappaB promotes Schwann cell differentiation into a myelinating phenotype." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 14, pp. 3738-46, 2008 (PubMed).

Ghosh, Yang, Rothstein et al.: "Nuclear factor-κB contributes to neuron-dependent induction of glutamate transporter-1 expression in astrocytes." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 25, pp. 9159-69, 2011 (PubMed).

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