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Details for Product No. ABIN1043728

Programmed Cell Death 4 (Neoplastic Transformation Inhibitor) (PDCD4) (pSer457) antibody

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Antigen
Synonyms H731, Ma3, Tis, D19Ucla1, Dug, h731, MGC69337, PDCD4
Epitope
»Alternatives pSer457
Reactivity
»Alternatives Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Pubmed 2 references available
Catalog no. ABIN1043728
Quantity 1 mg
Price
2,209.90 $   Plus shipping costs $45.00 and if applicable $26.40 dry ice
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Availability Will be delivered in 2 to 3 Business Days
Immunogen Anti-Pdcd4 phospho S457 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.
Immunogen Type: Peptide
Clone 2E10.C12
Isotype IgG1 kappa
Specificity Pdcd4 phospho S457 Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human Pdcd4 protein phosphorylated at Ser457.  A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence.  Cross-reactivity with Pdcd4 from other sources has not been determined.
Characteristics Programmed cell death 4 (Pdcd4) is a novel tumor supressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphoryl-ation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun. Ideal for researchers involved in Cell Signaling, Cancer and Signal Transduction research.
Sterility Sterile filtered
Alternative Name Pdcd4
Background Programmed cell death 4 (Pdcd4) is a novel tumor supressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphoryl-ation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun. Ideal for researchers involved in Cell Signaling, Cancer and Signal Transduction research.
Synonyms: Death up-regulated gene protein antibody, Dug antibody, H731 antibody, Ma3 antibody, Neoplastic transformation inhibitor antibody, Neoplastic transformation inhibitor protein antibody, Nuclear antigen H731 antibody
Gene ID 27250
NCBI Accession 21735596
UniProt Q53EL6
Application Notes This monoclonal antibody is suitable for ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 62 kDa in size corresponding to phosphorylated Pdcd4 protein by western blotting in the appropriate cell lysate or extract.  This phospho-specific monoclonal antibody reacts with human Pdcd4 pS457 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Comment

Gene Name: PDCD4

Restrictions For Research Use only
Format Liquid
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  Expiration date is one (1) year from date of opening.
Expiry Date 12 months
Product cited in: Jansen, Camalier, Colburn: "Epidermal expression of the translation inhibitor programmed cell death 4 suppresses tumorigenesis." in: Cancer research, Vol. 65, Issue 14, pp. 6034-41, 2005 (PubMed).

Huang, Wu, Loeb et al.: "Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion." in: The Journal of biological chemistry, Vol. 284, Issue 27, pp. 18515-24, 2009 (PubMed).

Alternatives for antigen "Programmed Cell Death 4 (Neoplastic Transformation Inhibitor) (PDCD4)", type "Antibodies"
Hosts (97), (19), (4)
Reactivities (125), (75), (74), (12), (6), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applications (106), (75), (38), (35), (11), (10), (10), (5), (4), (3), (2), (2)
Conjugates (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (20), (19), (9), (9), (6), (6), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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