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IL-3 antibody (HRP)

IL-3 Reactivity: Human WB, ELISA Host: Rabbit Polyclonal HRP
Catalog No. ABIN1043802
  • Target See all IL-3 Antibodies
    IL-3 (Interleukin 3 (IL-3))
    Reactivity
    • 72
    • 36
    • 23
    • 4
    Human
    Host
    • 75
    • 21
    • 17
    • 2
    • 2
    Rabbit
    Clonality
    • 81
    • 36
    Polyclonal
    Conjugate
    • 66
    • 17
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This IL-3 antibody is conjugated to HRP
    Application
    • 87
    • 43
    • 26
    • 26
    • 21
    • 12
    • 11
    • 11
    • 10
    • 10
    • 10
    • 9
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Specificity
    Anti-Human IL-3 (RABBIT) Antibody Peroxidase Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-3 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
    Characteristics
    The pleiotropic IL3 (Interleukin 3) is a 15kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL3 has also been shown to protect mast cells from undergoing apoptosis.
    Immunogen
    Anti-Human IL-3 (RABBIT) Antibody Peroxidase Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-3 protein.
    Immunogen Type: RecombinantProtein
    Isotype
    IgG
    Top Product
    Discover our top product IL-3 Primary Antibody
  • Application Notes
    This IL-3 antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 15 kDa in size corresponding to human IL-3 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
    Comment

    Gene Name: IL3

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Preservative
    Gentamicin sulfate
    Handling Advice
    Do NOT add Sodium Azide!
    Storage
    4 °C/-20 °C
    Storage Comment
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
    Expiry Date
    12 months
  • Davila, Froeling, Tan, Bonnard, Boland, Snippe, Hibberd, Seielstad: "New genetic associations detected in a host response study to hepatitis B vaccine." in: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, (2010) (PubMed).

  • Target
    IL-3 (Interleukin 3 (IL-3))
    Alternative Name
    IL-3 (IL-3 Products)
    Synonyms
    BPA antibody, Csfmu antibody, HCGF antibody, Il-3 antibody, MCGF antibody, PSF antibody, IL-3 antibody, MULTI-CSF antibody, interleukin 3 antibody, interleukin 3 (colony-stimulating factor, multiple) antibody, Il3 antibody, IL3 antibody
    Background
    The pleiotropic IL3 (Interleukin 3) is a 15kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL3 has also been shown to protect mast cells from undergoing apoptosis.
    Synonyms: Burst promoting activity, Colony stimulating factor, Eosinophil colony stimulating factor, Hematopoietic growth factor, IL 3, Interleukin 3
    Gene ID
    3562
    NCBI Accession
    NP_000579
    UniProt
    P08700
    Pathways
    JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
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