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Epstein-Barr Virus Induced 3 (EBI3) antibody
|1 reference available|
|Quantity||100 µg (1.0 mg/mL) (Variants)|
|Price||345.40 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
Anti-EBI-3 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant mouse EBI-3 produced in E.coli followed by hybridoma development.
Immunogen Type: Recombinant Protein
Anti-Mouse EBI-3 antibody has been tested for use in ELISA, IP, and Neutralization. Specific conditions for reactivity should be optimized by the end user.
Synonyms: Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein.
|Characteristics||The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-27 p28 or IL-12 p35 subunit. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R.|
|Specificity||Anti-Mouse EBI-3 (MOUSE) Monoclonal Antibody was purified from mouse ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse EBI3 protein. A BLAST analysis was used to suggest cross-reactivity with EBI3 from mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with EBI-3 from other sources has not been determined.|
Gene Name: EBI3
ELISA Dilution: 1:10,000.
Immunoprecipitation Dilution: 1µg/mL.
Western Blot Dilution: 1:1000.
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Preservative||0.01% (w/v) Sodium Azide|
|Storage Comment||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Expiry Date||Expiration date is one (1) year from date of opening.|
|Restrictions||For Research Use only|
|ELISA - EBI-3 antibody. Supernatant from 293T cells transfected with a vector containing murine IL-35 was added to an ELISA plate coated with V1.4H6.29 at 2ug/ml in PBS. mEBI3 was detected with biotinylated V1.4C4.22|
Collison, Workman, Kuo et al.: "The inhibitory cytokine IL-35 contributes to regulatory T-cell function." in: Nature, Vol. 450, Issue 7169, pp. 566-9, 2007 (PubMed).
|Hosts||Rabbit (39), Mouse (16), Rat (13), Goat (3)|
|Reactivities||Mouse (Murine) (44), Human (34), Rat (Rattus) (8), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1), Monkey (1)|
|Applications||Western Blotting (WB) (59), ELISA (48), Immunohistochemistry (IHC) (18), Flow Cytometry (FACS) (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Fluorescence Microscopy (FM) (7), Immunoprecipitation (IP) (7), Neutralization (Neut) (4), Immunofluorescence (IF) (2), Immunocytochemistry (ICC) (1)|
|Conjugates||Biotin (11), HRP (2), FITC (1)|
|Epitopes||Internal Region (6), C-Term (5), Middle Region (2), AA 1-230 (1), AA 120-170 (1), AA 200-230 (1), AA 50-100 (1), AA 51-67 (1), C-Term,AA 153-181 (1), N-Term (1)|