Epstein-Barr Virus Induced 3 (EBI3) antibody

Details for Product No. ABIN1043837
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Antigen
Synonyms EBI-3, IL-27, IL-27B, IL27B
Reactivity
Mouse (Murine)
(65), (57), (22), (12), (12), (12), (1)
Host
Rat
(59), (21), (13), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(13), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), ELISA, Flow Cytometry (FACS)
(77), (57), (20), (18), (15), (10), (10), (10), (7), (3), (2), (2)
Pubmed 1 reference available
Catalog no. ABIN1043837
Quantity 100 µg
Price
361.90 $   Plus shipping costs $45.00 and if applicable $26.40 dry ice
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Immunogen Anti-EBI-3 (RAT) Monoclonal Antibody was produced in rat by repeated immunizations with mature full length recombinant mouse EBI-3 produced in E.coli followed by hybridoma development.
Immunogen Type: Recombinant Protein
Clone 10J811
Isotype IgG
Specificity This product was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse EBI3 protein. A BLAST analysis was used to suggest cross-reactivity with EBI3 from mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with EBI-3 from other sources has not been determined.
Characteristics The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-27 p28 or IL-12 p35 subunit. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R.
Sterility Sterile filtered
Alternative Name EBI-3
Background The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-27 p28 or IL-12 p35 subunit. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R.
Synonyms: Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein
Gene ID 50498
NCBI Accession NP_056581.1
UniProt O35228
Application Notes Anti-EBI-3 antibody has been tested for use in ELISA, Western Blot, and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.
Comment

Gene Name: EBI3

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  Expiration date is one (1) year from date of opening.
Expiry Date 12 months
Supplier Images
anti-Epstein-Barr Virus Induced 3 (EBI3) antibody Flow Cytometry of Anti-Mouse EBI3 Antibody: Figure A: Intracellular flow cytometry of mouse EBI3 in transfected and untransfected HEK 293 cells (Brefeldin A treated, 5 hours) using mouse EBI3 antibody at 3 ug/10^6 cells. Figure B: Intracellular flow cytometry of mouse EBI3 in stimulated and unstimulated RAW cells (LPS treated, 50 ng/ml, overnight) using mouse EBI3 antibody at 1.5 ug/10^6 cells.
Product cited in: Collison, Workman, Kuo et al.: "The inhibitory cytokine IL-35 contributes to regulatory T-cell function." in: Nature, Vol. 450, Issue 7169, pp. 566-9, 2007 (PubMed).

Hosts (59), (21), (13), (3)
Reactivities (65), (57), (22), (12), (12), (12), (1)
Applications (77), (57), (20), (18), (15), (10), (10), (10), (7), (3), (2), (2)
Conjugates (13), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (9), (5), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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