Hemagglutinin (HA) antibody

Antigen: Hemagglutinin (HA)

Protein Type: Native

Clonality: Monoclonal (14C5)

Host: Mouse

Reactivity: Influenza A Virus H5N1

Conjugate: Un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Fluorescence Microscopy (FM), Western Blotting (WB)

Catalog no.: ABIN1043852

Additional Information

NCBI Acession: 159144921

UniProt: A8UDQ2

Immunogen: This monoclonal antibody was produced by intraperitoneal immunization of BALB/c mice with concentrated purified virus preparation containing hemagglutinin (HA) protein of influenza A virus [strain A/Vietnam/1203/04 (H5N1)] using the modification of the method described by Kohler and Milstein. Each mouse received two immunizations of 15 µg HA with incomplete Freund’s adjuvant, administered 3 week apart.
Immunogen Type: Native Protein

Format: Liquid

Isotype: IgG2a kappa

Clone: 14C5

Description: This monoclonal antibody can be used for hemagglutination inhibition (HI) assays to provide antigenic characterization of the influenza A viruses of the H5 HA subtype. This monoclonal antibody is suitable for virus neutralization assays (in cell culture and in embryonated chicken eggs), ELISA, immunoprecipitation, immunohisto-chemistry and western blotting.

Synonyms: H5HA antibody, Hemagglutinin 5 antibody, H5N1 antibody.

Characteristics: Antibody raised against the hemagglutinin (HA) surface glycoprotein of the A/Vietnam/1203/04 (H5N1) influenza virus. Generally referred to as ''bird flu'', the H5N1 influenza A virus has been documented in poultry and humans across ten Eurasian countries, from Japan in the north to Indonesia in the south. Without immunity, humans would have no protection against H5N1 influenza viruses, which could potentially cause a catastrophic pandemic influenza.  This antibody, directed against the HA surface glycoprotein of the A/Vietnam/1203/04 (H5N1) influenza virus, is intended to further our understanding of the mechanisms underlying antigenic variation and evolution of novel variants. The major functions of HA include receptor-binding and fusion activities, but there may also be a structural role for HA in viral particle formation. Following attachment of HA to surface receptors on susceptible cells, the influenza virus enters the cell via endocytosis and membrane fusion.

Specificity: This product was clarified from mouse ascitic fluid and is specific for H5 hemagglutinin (HA) protein of influenza A virus [strain A/Vietnam/1203/04 (H5N1)].  VN04-13 monoclonal antibody did not cross-react with influenza viruses of other HA subtypes. This monoclonal antibody reacted with H5N1 influenza virus representatives of different clades and subclades of the H5 HA subtype.

Comments:

Gene Name: HA

Sterility: sterile filtered

Application Details

Application Notes: ELISA Dilution: 1:5,000.

Preservative: 0.01% (w/v) Sodium Azide

Storage: 4°C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: Expiration date is one (1) year from date of opening.

Restrictions: For Research Use only

Images

Shown are titers of anti H5N1 antibodies against a variety of H5N1 influenza viruses .

Publications

Product: Guan, Poon, Cheung et al.: "H5N1 influenza: a protean pandemic threat." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 21, pp. 8156-61, 2004 (PubMed).

Hoffmann, Lipatov, Webby et al.: "Role of specific hemagglutinin amino acids in the immunogenicity and protection of H5N1 influenza virus vaccines." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 36, pp. 12915-20, 2005 (PubMed).