Collagen, Type III, alpha 1 (COL3A1) antibody

Antigen: Collagen, Type III, alpha 1 (COL3A1)

Synonyms: EDS4A, FLJ34534, Ms10w, Col3a-1, MMS10-W, AW550625, KIAA4231, mKIAA4231, MGC93704, COL3A1, MGC139882, LOC100220437

Protein Type: Native

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine)

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN1043861

Additional Information

Alternative name: Collagen Type III

Gene ID: 1281

NCBI Acession: P02461.4

UniProt: P02461

Immunogen: Collagen Type III from human and bovine placenta
Immunogen Type: Native Protein

Format: Liquid

Isotype: IgG

Description: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for native (non-denaturing, non-dissociating) PAGE and western blotting for highly sensitive qualitative analysis.

Synonyms: Collagen type III alpha 1 antibody, Collagen type III alpha antibody, EDS4A antibody, Ehlers Danlos syndrome type IV, autosomal dominant antibody, Fetal collagen antibody.

Characteristics: Highly active antibodies and conjugates to collagens. Collagens areHighly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of ‘type’ specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Extensively purified collagens are used for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Ideal for investigators involved in Cell Biology, Signal Transduction and Stem Cell research.

Specificity: Collagen III Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type III collagens and has negligible cross-reactivity with Type I, II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Sterility: sterile filtered

Application Details

Application Notes: ELISA Dilution: 1:5,000 - 1:50,000.
Immunohistochemistry Dilution: 1:50 - 1:200.
Immunoprecipitation Dilution: 1:100.
Western Blot Dilution: 1:1,000 - 1:10,000.

Concentration: 1.0 mg/mL

Purification: Antiserum

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Preservative: 0.01% (w/v) Sodium Azide

Storage: 4°C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Anti-Mouse IgG HRP secondary antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: Expiration date is one (1) year from date of opening.

Research Area: Extracellular Matrix

Restrictions: For Research Use only

Images

anti collagen III antibody (, 1:400, 45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA

anti-Collagen, Type III, alpha 1 (COL3A1) antibody (Image 2)

Publications

Product: Välkkilä, Melkoniemi, Kvist et al.: "Genomic organization of the human COL3A1 and COL5A2 genes: COL5A2 has evolved differently than the other minor fibrillar collagen genes." in: Matrix biology : journal of the International Society for Matrix Biology, Vol. 20, Issue 5-6, pp. 357-66, 2001 (PubMed).

Cairns, Harris, Mak: "Regulation of cancer cell metabolism." in: Nature reviews. Cancer, Vol. 11, Issue 2, pp. 85-95, 2011 (PubMed).