Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (Internal Region) antibody

Antigen: Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1)

Synonyms: TM, EAT, MCL1L, MCL1S, Mcl-1, BCL2L3, MGC1839, MGC104264

Binding Site: Internal Region

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN1043872

Additional Information

Alternative name: Mcl-1

Gene ID: 17210

NCBI Acession: P97287.3

UniProt: P97287

Immunogen: This affinity purified antibody was purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Mcl-1 conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Peptide

Format: Liquid

Isotype: IgG

Description: This antibody is suitable for western blotting, immunoprecipitation, and ELISA. This antibody detects mouse Mcl-1 and is not expected to cross react with the human sequence. Cross reactivity with Mcl-1 from other sources is unknown. This antibody is highly specific, showing no bands in null cell lysates from Mcl-1 knockout mice even when grossly over-exposed. Mouse Mcl-1 (myeloid cell leukemia sequence 1) is composed of 331 amino acids and is reported to be 35.2 kDa in size. The human ortholog consists of 351 amino acids and is reported to be 37.3 kDa in size.

Synonyms: Bcl 2 related protein EAT/mcl1 antibody, BCL2 related antibody, BCL2L3 antibody, EAT antibody, Induced myeloid leukemia cell differentiation protein Mcl 1 antibody.

Characteristics: Regulated apoptosis is essential for both the development and the subsequent maintenance of the immune system. Interleukins, including IL-2, IL-4, IL-7 and IL-15, heavily influence lymphocyte survival during the vulnerable stages of VDJ rearrangement and later in ensuring cellular homeostasis, but the genes specifically responsible for the development and maintenance of lymphocytes have not been identified. The Anti apoptotic protein Mcl-1 (myeloid cell leukemia sequence 1 (BCL2-related)) is an attractive candidate, as it is highly regulated, appears to enhance short-term survival and functions at an apical step in genotoxic deaths. However, Mcl-1 deficiency results in peri-implantation lethality. Mice, conditional for Mcl-1, display a profound reduction in B and T lymphocytes when Mcl-1 is removed. Deletion of Mcl-1 during early lymphocyte differentiation increases apoptosis and arrests the development at pro-B-cell and double negative T-cell stages. Induced deletion of Mcl-1 in peripheral B- and T-cell populations results in their rapid loss. Moreover, IL-7 both induces and requires Mcl-1 to mediate lymphocyte survival. Mcl-1 is essential both early in lymphoid development and later on in the maintenance of mature lymphocytes.

Specificity: This affinity-purified antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads followed by cross adsorption to remove any unwanted reactivity. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. No reaction was observed against KLH.

Comments:

Gene Name: MCL-1

Sterility: sterile filtered

Application Details

Application Notes: ELISA Dilution: 1:10,000 - 1:50,000.
Western Blot Dilution: 1:10,000.

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.1% (w/v) Sodium Azide

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: Expiration date is one (1) year from date of opening.

Research Area: Cancer, Apoptosis/Necrosis

Restrictions: For Research Use only

Images

Western blot analysis using Immuno-chemical's anti-Mcl-1 antibody to detect Mcl-1 in MEF cell lysates (20 µg per lane). Anti-Mcl-1 was used at a dilution of 1:10,000 followed by reaction with HRP Anti-Rabbit IgG [H&L] MX10 (GOAT) used at a 1:2,000 dilution. Western Lightning Chemiluminescence Reagent Plus from Perkin Elmer was used for detection. The exposure time was exactly 30 seconds. This antibody detects 35 kDa mouse Mcl-1. Communicated with permission by J. Opferman and S. Korsmeyer. See Opferman et al (2003) for additional details.

Publications

Product: Craig, Jabs, Zhou et al.: "Human and mouse chromosomal mapping of the myeloid cell leukemia-1 gene: MCL1 maps to human chromosome 1q21, a region that is frequently altered in preneoplastic and neoplastic disease." in: Genomics, Vol. 23, Issue 2, pp. 457-63, 1995 (PubMed).

Li, Krajewski, Reed et al.: "The apoptosis and proliferation of SAC-activated B cells by IL-10 are associated with changes in Bcl-2, Bcl-xL, and Mcl-1 expression." in: Cellular immunology, Vol. 178, Issue 1, pp. 33-41, 1997 (PubMed).

Opferman, Letai, Beard et al.: "Development and maintenance of B and T lymphocytes requires antiapoptotic MCL-1." in: Nature, Vol. 426, Issue 6967, pp. 671-6, 2003 (PubMed).