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Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (Internal Region) antibody
|Synonyms||TM, EAT, MCL1L, MCL1S, Mcl-1, BCL2L3, MGC1839, MGC104264|
Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
|3 references available|
|Quantity||25 µL (1.0 mg/mL) (Variants)|
|Price||119.90 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
This affinity purified antibody was purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Mcl-1 conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Peptide
This antibody is suitable for western blotting, immunoprecipitation, and ELISA. This antibody detects mouse Mcl-1 and is not expected to cross react with the human sequence. Cross reactivity with Mcl-1 from other sources is unknown. This antibody is highly specific, showing no bands in null cell lysates from Mcl-1 knockout mice even when grossly over-exposed. Mouse Mcl-1 (myeloid cell leukemia sequence 1) is composed of 331 amino acids and is reported to be 35.2 kDa in size. The human ortholog consists of 351 amino acids and is reported to be 37.3 kDa in size.
Synonyms: Bcl 2 related protein EAT/mcl1 antibody, BCL2 related antibody, BCL2L3 antibody, EAT antibody, Induced myeloid leukemia cell differentiation protein Mcl 1 antibody.
|Characteristics||Regulated apoptosis is essential for both the development and the subsequent maintenance of the immune system. Interleukins, including IL-2, IL-4, IL-7 and IL-15, heavily influence lymphocyte survival during the vulnerable stages of VDJ rearrangement and later in ensuring cellular homeostasis, but the genes specifically responsible for the development and maintenance of lymphocytes have not been identified. The Anti apoptotic protein Mcl-1 (myeloid cell leukemia sequence 1 (BCL2-related)) is an attractive candidate, as it is highly regulated, appears to enhance short-term survival and functions at an apical step in genotoxic deaths. However, Mcl-1 deficiency results in peri-implantation lethality. Mice, conditional for Mcl-1, display a profound reduction in B and T lymphocytes when Mcl-1 is removed. Deletion of Mcl-1 during early lymphocyte differentiation increases apoptosis and arrests the development at pro-B-cell and double negative T-cell stages. Induced deletion of Mcl-1 in peripheral B- and T-cell populations results in their rapid loss. Moreover, IL-7 both induces and requires Mcl-1 to mediate lymphocyte survival. Mcl-1 is essential both early in lymphoid development and later on in the maintenance of mature lymphocytes.|
|Specificity||This affinity-purified antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads followed by cross adsorption to remove any unwanted reactivity. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. No reaction was observed against KLH.|
Gene Name: MCL-1
ELISA Dilution: 1:10,000 - 1:50,000.
Western Blot Dilution: 1:10,000.
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Preservative||0.1% (w/v) Sodium Azide|
|Storage Comment||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Expiry Date||Expiration date is one (1) year from date of opening.|
|Research Area||Cancer, Apoptosis/Necrosis|
|Restrictions||For Research Use only|
Craig, Jabs, Zhou et al.: "Human and mouse chromosomal mapping of the myeloid cell leukemia-1 gene: MCL1 maps to human chromosome 1q21, a region that is frequently altered in preneoplastic and neoplastic disease." in: Genomics, Vol. 23, Issue 2, pp. 457-63, 1995 (PubMed).
Li, Krajewski, Reed et al.: "The apoptosis and proliferation of SAC-activated B cells by IL-10 are associated with changes in Bcl-2, Bcl-xL, and Mcl-1 expression." in: Cellular immunology, Vol. 178, Issue 1, pp. 33-41, 1997 (PubMed).
Opferman, Letai, Beard et al.: "Development and maintenance of B and T lymphocytes requires antiapoptotic MCL-1." in: Nature, Vol. 426, Issue 6967, pp. 671-6, 2003 (PubMed).