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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
GLI Family Zinc Finger 2 (GLI2) (Internal Region) antibody
|Synonyms||HPE9, THP1, THP2, AW546128, GLI2, GLI2/GLI4|
Alternatives ELISA, Fluorescence Microscopy (FM), Western Blotting (WB)
|1 reference available|
|Quantity||25 µL (Variants)|
|Price||119.90 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 46-60 of human Gli-2 (isoform a).
Immunogen Type: Peptide
This antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. See figure legend for expectations by western blot. Multiple splice variants have been reported for this protein a, b, g and d (133.3, 131.6, 88.1 and 86.4 kDa respectively). Detection of Gli-2 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-2 is likely to be low. Furthermore, Gli-2 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.
Synonyms: Gli-2, Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein.
|Characteristics||Gli-2 (also known as Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein) belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila.|
|Specificity||This affinity-purified antibody is directed against human Gli-2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Gli-2 from human and chimpanzee based on the immunizing sequence.|
Gene Name: GLI2
ELISA Dilution: 1:2,000 - 1:12,000.
Immunohistochemistry Dilution: 1:500 - 1:2,000.
Western Blot Dilution: 1:500 - 1:2,000.
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Preservative||0.01% (w/v) Sodium Azide|
|Storage Comment||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Expiry Date||Expiration date is one (1) year from date of opening.|
|Research Area||Growth Factors|
|Restrictions||For Research Use only|
Roessler, Ermilov, Grange et al.: "A previously unidentified amino-terminal domain regulates transcriptional activity of wild-type and disease-associated human GLI2." in: Human molecular genetics, Vol. 14, Issue 15, pp. 2181-8, 2005 (PubMed).
|Hosts||Rabbit (34), Goat (4), Sheep (1)|
|Reactivities||Human (30), Mouse (Murine) (29), Rat (Rattus) (25), Cow (Bovine) (21), Dog (Canine) (19), Horse (Equine) (18), Pig (Porcine) (18), Rabbit (18), Chimpanzee (2), Cat (Feline) (1), Chicken (1)|
|Applications||Western Blotting (WB) (21), ELISA (19), Immunofluorescence (IF) (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Immunohistochemistry (IHC) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoprecipitation (IP) (2), Chromatin Immunoprecipitation (ChiP) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)|
|Epitopes||Internal Region (4), AA 46-60 (1), Isoform A,AA 46-60 (1), N-Term (1)|