GLI Family Zinc Finger 2 (GLI2) (Internal Region) antibody

Antigen: GLI Family Zinc Finger 2 (GLI2)

Synonyms: HPE9, THP1, THP2, AW546128, GLI2, GLI2/GLI4

Binding Site: Internal Region

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Chimpanzee

Conjugate: Un-conjugated

Application: ELISA, Fluorescence Microscopy (FM), Western Blotting (WB)

Catalog no.: ABIN1043881

Additional Information

Alternative name: Gli-2

Gene ID: 2736

NCBI Acession: NP_005261.2

UniProt: P10070

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 46-60 of human Gli-2 (isoform a).
Immunogen Type: Peptide

Format: Liquid

Isotype: IgG

Description: This antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. See figure legend for expectations by western blot. Multiple splice variants have been reported for this protein a, b, g and d (133.3, 131.6, 88.1 and 86.4 kDa respectively). Detection of Gli-2 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-2 is likely to be low. Furthermore, Gli-2 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.

Synonyms: Gli-2, Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein.

Characteristics: Gli-2 (also known as Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein) belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila.

Specificity: This affinity-purified antibody is directed against human Gli-2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Gli-2 from human and chimpanzee based on the immunizing sequence.

Comments:

Gene Name: GLI2

Sterility: sterile filtered

Application Details

Application Notes: ELISA Dilution: 1:2,000 - 1:12,000.
Immunohistochemistry Dilution: 1:500 - 1:2,000.
Western Blot Dilution: 1:500 - 1:2,000.

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Storage: 4°C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: Expiration date is one (1) year from date of opening.

Research Area: Growth Factors

Restrictions: For Research Use only

Images

Western blot using affinity purified anti-Gli-2 antibody shows detection of Gli-2 protein in rat testes (lane 1) and human HEK293 (lane 2) whole cell lysates (arrowhead). See Ruppert et al for testing conditions. Each lane contains approximately 35 µg of lysate. Primary antibody was used at a 1:400 dilution in 5% BLOTTO in PBS overnight at 4°C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 (611-132-122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

anti-GLI Family Zinc Finger 2 (GLI2) (Internal Region) antibody (Image 2)

Publications

Product: Roessler, Ermilov, Grange et al.: "A previously unidentified amino-terminal domain regulates transcriptional activity of wild-type and disease-associated human GLI2." in: Human molecular genetics, Vol. 14, Issue 15, pp. 2181-8, 2005 (PubMed).