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RPS6KA1 antibody (C-Term)

RPS6KA1 Reactivity: Human, Mouse, Rat, Cow, Dog, Horse WB, ELISA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1043904
  • Target See all RPS6KA1 Antibodies
    RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))
    Binding Specificity
    • 41
    • 30
    • 25
    • 18
    • 17
    • 16
    • 15
    • 8
    • 8
    • 6
    • 6
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 206
    • 123
    • 91
    • 7
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Cow, Dog, Horse
    Host
    • 216
    • 17
    Rabbit
    Clonality
    • 193
    • 41
    Polyclonal
    Conjugate
    • 107
    • 17
    • 14
    • 11
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 7
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    This RPS6KA1 antibody is un-conjugated
    Application
    • 185
    • 104
    • 54
    • 53
    • 50
    • 27
    • 25
    • 25
    • 15
    • 12
    • 9
    • 3
    • 2
    • 1
    Western Blotting (WB), ELISA
    Specificity
    Anti-p90 RSK1 was affinity purified from monospecific antiserum by immunoaffinity chromatography using phosphorylated peptide coupled to agarose beads followed by solid phase adsorption against non phosphorylated peptide. This antibody is specific for human p90 RSK1 protein. A BLAST analysis was used to suggest cross reactivity with p90 RSK1 from human, rat, mouse, horse, bovine, and dog based on 100% homology with the immunizing sequence. Cross reactivity with p90 RSK1 pS732 from other sources has not been determined.
    Characteristics
    Ribosomal S6 Kinase 1 (RSK1) is an immediate downstream effector of mitogen activated protein kinases and therefore promotes cell proliferation and survival. It has serine/threonine kinase activity and may play a role in mediating the growth-factor and stress-induced activation of the transcription factor CREB. The C-terminal region of RSK1 is reported to be an ERK docking site, where serine 732 phosphorylation status is critical for RSK1 activation. When serine 732 is not phosphorylated, ERK1/2 binds to the ERK docking site of RSK1, and upon stimulation, activates RSK1. The activated RSK1 then autophosphorylates serine 732, leading to the dissociation of ERK from RSK1 and termination of activation by ERK. RSK1 is studied in cancer research and is known to inactive tumor supressor complexes and death kinases.
    Sterility
    Sterile filtered
    Immunogen
    Anti-p90 RSK1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal end of human RSK1 protein.
    Immunogen Type: Peptide
    Isotype
    IgG
    Top Product
    Discover our top product RPS6KA1 Primary Antibody
  • Application Notes
    Anti-p90 RSK1 antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot a band approximately 90 kDa in size corresponding to p90 RSK1 protein is expected in the appropriate cell lysate or extract.
    Comment

    Gene Name: RPS6KA1

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.51 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
    Expiry Date
    12 months
  • Chaturvedi, Poppleton, Stringfield, Barbier, Patel: "Subcellular localization and biological actions of activated RSK1 are determined by its interactions with subunits of cyclic AMP-dependent protein kinase." in: Molecular and cellular biology, Vol. 26, Issue 12, pp. 4586-600, (2006) (PubMed).

  • Target
    RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))
    Alternative Name
    p90 RSK1 (RPS6KA1 Products)
    Synonyms
    HU-1 antibody, MAPKAPK1A antibody, RSK antibody, RSK1 antibody, Rsk1 antibody, p90rsk antibody, rsk antibody, RPS6KA antibody, hu-1 antibody, mapkapk1a antibody, rsk1 antibody, wu:fc56h07 antibody, zgc:152654 antibody, ribosomal protein S6 kinase A1 antibody, ribosomal protein S6 kinase polypeptide 1 antibody, ribosomal protein S6 kinase A1-like antibody, ribosomal protein S6 kinase A1 L homeolog antibody, ribosomal protein S6 kinase a, polypeptide 1 antibody, RPS6KA1 antibody, Rps6ka1 antibody, RPS6KA1L antibody, rps6ka1.L antibody, rps6ka1 antibody
    Background
    Ribosomal S6 Kinase 1 (RSK1) is an immediate downstream effector of mitogen activated protein kinases and therefore promotes cell proliferation and survival. It has serine/threonine kinase activity and may play a role in mediating the growth-factor and stress-induced activation of the transcription factor CREB. The C-terminal region of RSK1 is reported to be an ERK docking site, where serine 732 phosphorylation status is critical for RSK1 activation. When serine 732 is not phosphorylated, ERK1/2 binds to the ERK docking site of RSK1, and upon stimulation, activates RSK1. The activated RSK1 then autophosphorylates serine 732, leading to the dissociation of ERK from RSK1 and termination of activation by ERK. RSK1 is studied in cancer research and is known to inactive tumor supressor complexes and death kinases.
    Synonyms: p90 RSK1, p90-RSK 1, p90RSK1, p90S6K, Ribosomal S6 Kinase 1, RSK1, S6K-alpha 1, 90 kDa Ribosomal Protein S6 Kinase 1, MAP kinase-activated protein kinase 1a, MAPKAPK1A, MAPKAP kinase 1a, MAPKAPK-1a
    Gene ID
    6195
    NCBI Accession
    NP_001006666
    UniProt
    Q15418
    Pathways
    MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
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