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alpha Tubulin antibody (TUBA1) (AA 427-441) (Biotin)

Details for Product anti-TUBA1 Antibody No. ABIN1043919, Supplier: Log in to see
Antigen
  • K-ALPHA-1
  • TUBA2
  • mec-12
  • tuba4
Alternatives
anti-Human alpha Tubulin antibody for Western Blotting
Epitope
AA 427-441
15
14
5
4
4
3
3
2
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Chicken, Cow (Bovine), Goat, Sheep (Ovine)
105
62
55
27
25
18
13
11
9
8
7
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
93
39
16
6
2
Clonality
Polyclonal
Conjugate
This alpha Tubulin antibody is conjugated to Biotin
7
5
4
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
134
46
44
43
42
41
39
15
9
6
2
1
1
Supplier
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Immunogen Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 427-441 of Human alpha Tubulin.
Isotype IgG
Specificity Anti-Tubulin Loading Control Antibody is directed against human alpha Tubulin protein.  The Loading Control Antibody was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
Characteristics Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control.
Alternative Name Alpha-Tubulin (TUBA1 Antibody Abstract)
Background Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.  This antibody makes an excellent loading control.
Synonyms: Loading Control Antibody
Pathways Microtubule Dynamics
Application Notes Anti-Tubulin Loading Control Antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in the appropriate cell lysate or extract.
Comment

Gene Name: TUBA1B

Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Concentration 0.61 mg/ml
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months
Product cited in: Hall, Cowan: "Structural features and restricted expression of a human alpha-tubulin gene." in: Nucleic acids research, Vol. 13, Issue 1, pp. 207-23, 1985 (PubMed).