Progesterone Receptor (PGR) (Internal Region) antibody

Antigen: Progesterone Receptor (PGR)

Synonyms: PR, NR3C3, PR-A, PR-B, BB114106, 9930019P03Rik, ENSMUSG00000074510, PGR, pgr-A, XPR-1, LOC443444, pg, pr, gb:dq017620

Binding Site: Internal Region

Protein Type: Recombinant

Clonality: Monoclonal (SP2)

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN1044091

Additional Information

Alternative name: Progesterone Receptor

Gene ID: 5241

NCBI Acession: P06401.4

UniProt: P06401

Immunogen: Anti-Progesterone Receptor monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues 412-526 of human Progesterone Receptor protein. The hybridoma was produced by fusing New Zealand White rabbit splenocytes and myeloma cells using conventional technology.
Immunogen Type: Recombinant Protein

Format: Liquid

Isotype: IgG

Clone: SP2

Description: This monoclonal antibody is suitable for ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 99 kDa in size corresponding to PgR isoform B (the ‘canonical’ sequence) protein by western blotting in the appropriate cell lysate or extract. Progesterone Receptor is found in human breast carcinoma tissue as a nuclear protein. For immunohistochemistry, samples should be formalin fixed and paraffin embedded. Deparaffinize slides using xylene or xylene alternatives and graded alcohols. Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min.

Synonyms: PgR PR nuclear receptor subfamily 3 group C member 3.

Characteristics: The PGR gene encodes a member of the steroid receptor superfamily referred to as progesterone receptor (also known as PgR, PR and nuclear receptor subfamily 3 group C member 3). Progesterone receptor mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform A only, and mediate their own response genes and physiologic effects with little overlap. Ideal for Cancer, Chromatin & Nuclear Signaling and Signal Transduction research.

Specificity: This product is clarified tissue culture supernate. The antibody is specific for human Progesterone Receptor protein. Cross-reactivity with Progesterone Receptor protein from other sources has not been determined.

Comments:

Gene Name: PGR

Sterility: sterile filtered

Application Details

Application Notes: ELISA Dilution: 1:10,000.
Immunohistochemistry Dilution: 1:400.
Western Blot Dilution: 1:200-1:500.

Purification: CCS

Preservative: 0.1% (w/v) Sodium Azide

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: Expiration date is six (6) months from date of opening.

Research Area: Cancer, Endocrine system, Transcription Factors, Hormones, Receptors

Restrictions: For Research Use only

Images

Progesterone Receptor (PR) monoclonal antibody stains Human Breast Carcinoma.

anti-Progesterone Receptor (PGR) (Internal Region) antibody (Image 2)

Publications

Product: Kastner, Krust, Turcotte et al.: "Two distinct estrogen-regulated promoters generate transcripts encoding the two functionally different human progesterone receptor forms A and B." in: The EMBO journal, Vol. 9, Issue 5, pp. 1603-14, 1990 (PubMed).

Cano, Milanezi, Leitão et al.: "Estimation of hormone receptor status in fine-needle aspirates and paraffin-embedded sections from breast cancer using the novel rabbit monoclonal antibodies SP1 and SP2." in: Diagnostic cytopathology, Vol. 29, Issue 4, pp. 207-11, 2003 (PubMed).