Myogenic Differentiation 1 (MYOD1) antibody

Antigen: Myogenic Differentiation 1 (MYOD1)

Synonyms: PUM, MYF3, MYOD, bHLHc1, MyoD, MGC143434, pum, myf3, XMyoD, XtmyoD, MGC75960, MUF3, Myod-1, AI503393, myod, MGC136744, wu:fb57a01, zgc:136744, etID309723.25, MGC156574, MYOD1, myod1

Clonality: Monoclonal (5-2F)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN104465

Additional Information

Alternative name: MyoD1

Immunogen: BALB/C mice were injected with a recombinant mouse MyoD1 protein.

Format: Supernatant (prediluted)

Isotype: IgG2a  (Matching secondary antibodies)

Clone: 5-2F

Specificity: This antibody is specific to a 45 kD protein, which is identified as MyoD1. This antibody is specific to an epitope of amino acid 3-56 in the N-terminus of mouse MyoD1. This antibody does not react with myogenin, Myf5 or Myf6. MyoD1 stains the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue but is expressed strongly in the tumor cell nuclei of rhabdomyosarcomas.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. This antibody is suitable for frozen tissues sections only. Positive Control: Tonsil. Cellular localization: Nuclear

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union