Lipocalin 2 antibody

Catalog No. ABIN1045905

Product Details anti-Lipocalin 2 Antibody

Target: Lipocalin 2 (LCN2)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lipocalin 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Specific for Lipocalin 2, native and denatured forms

Cross-Reactivity: Human, Mouse

Purification: Protein G purified

Immunogen: Recombinant Human Neutrophil gelatinase-associated lipocalin protein

Clone: 3B1C10

Isotype: IgG2b

Application Details

Application Notes: Recommended dilution:WB:1:500-1:5000,

Comment:

Other species are not tested. Please decide the specificity by homology

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid freeze and thaw cycles.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

References for anti-Lipocalin 2 antibody (ABIN1045905)

Wagener, Minhaz, Mattis, Kim, Emond, Lee: "Urinary neutrophil gelatinase-associated lipocalin as a marker of acute kidney injury after orthotopic liver transplantation." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 26, Issue 5, pp. 1717-23, (2011) (PubMed).

Prabhu, Sujatha, Ninan, Vijayalakshmi: "Neutrophil gelatinase associated lipocalin as a biomarker for acute kidney injury in patients undergoing coronary artery bypass grafting with cardiopulmonary bypass." in: Annals of vascular surgery, Vol. 24, Issue 4, pp. 525-31, (2010) (PubMed).

Devarajan: "Review: neutrophil gelatinase-associated lipocalin: a troponin-like biomarker for human acute kidney injury." in: Nephrology (Carlton, Vic.), Vol. 15, Issue 4, pp. 419-28, (2010) (PubMed).

Bagshaw, Bennett, Haase, Haase-Fielitz, Egi, Morimatsu, Damico, Goldsmith, Devarajan, Bellomo: "Plasma and urine neutrophil gelatinase-associated lipocalin in septic versus non-septic acute kidney injury in critical illness." in: Intensive care medicine, Vol. 36, Issue 3, pp. 452-61, (2010) (PubMed).

Kümpers, Hafer, Lukasz, Lichtinghagen, Brand, Fliser, Faulhaber-Walter, Kielstein: "Serum neutrophil gelatinase-associated lipocalin at inception of renal replacement therapy predicts survival in critically ill patients with acute kidney injury." in: Critical care, Vol. 14, Issue 1, pp. R9, (2010) (PubMed).

Makris, Markou, Evodia, Dimopoulou, Drakopoulos, Ntetsika, Rizos, Baltopoulos, Haliassos: "Urinary neutrophil gelatinase-associated lipocalin (NGAL) as an early marker of acute kidney injury in critically ill multiple trauma patients." in: Clinical chemistry and laboratory medicine : CCLM / FESCC, Vol. 47, Issue 1, pp. 79-82, (2009) (PubMed).

Yilmaz, Sevketoglu, Gedikbasi, Karyagar, Kiyak, Mulazimoglu, Aydogan, Ozpacaci, Hatipoglu: "Early prediction of urinary tract infection with urinary neutrophil gelatinase associated lipocalin." in: Pediatric nephrology (Berlin, Germany), Vol. 24, Issue 12, pp. 2387-92, (2009) (PubMed).

Tuladhar, Püntmann, Soni, Punjabi, Bogle: "Rapid detection of acute kidney injury by plasma and urinary neutrophil gelatinase-associated lipocalin after cardiopulmonary bypass." in: Journal of cardiovascular pharmacology, Vol. 53, Issue 3, pp. 261-6, (2009) (PubMed).

Devarajan: "Neutrophil gelatinase-associated lipocalin (NGAL): a new marker of kidney disease." in: Scandinavian journal of clinical and laboratory investigation. Supplementum, Vol. 241, pp. 89-94, (2008) (PubMed).

Bennett, Dent, Ma, Dastrala, Grenier, Workman, Syed, Ali, Barasch, Devarajan: "Urine NGAL predicts severity of acute kidney injury after cardiac surgery: a prospective study." in: Clinical journal of the American Society of Nephrology : CJASN, Vol. 3, Issue 3, pp. 665-73, (2008) (PubMed).

McCormick, Venn: "Recently published papers: Tracheostomy: why rather than when? Obesity: does it matter? And stroke: diagnosis, thrombosis and prognosis." in: Critical care, Vol. 11, Issue 2, pp. 127, (2008) (PubMed).

Dent, Ma, Dastrala, Bennett, Mitsnefes, Barasch, Devarajan: "Plasma neutrophil gelatinase-associated lipocalin predicts acute kidney injury, morbidity and mortality after pediatric cardiac surgery: a prospective uncontrolled cohort study." in: Critical care, Vol. 11, Issue 6, pp. R127, (2008) (PubMed).

Wolf: "Are oral contraceptives associated with increased renin-angiotensin activity and risk of nephropathy in diabetic women?" in: Nature clinical practice. Nephrology, Vol. 2, Issue 2, pp. 78-9, (2006) (PubMed).

Wagener, Jan, Kim, Mori, Barasch, Sladen, Lee: "Association between increases in urinary neutrophil gelatinase-associated lipocalin and acute renal dysfunction after adult cardiac surgery." in: Anesthesiology, Vol. 105, Issue 3, pp. 485-91, (2006) (PubMed).

Mishra, Dent, Tarabishi, Mitsnefes, Ma, Kelly, Ruff, Zahedi, Shao, Bean, Mori, Barasch, Devarajan: "Neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute renal injury after cardiac surgery." in: Lancet, Vol. 365, Issue 9466, pp. 1231-8, (2005) (PubMed).

Mishra, Mori, Ma, Kelly, Barasch, Devarajan: "Neutrophil gelatinase-associated lipocalin: a novel early urinary biomarker for cisplatin nephrotoxicity." in: American journal of nephrology, Vol. 24, Issue 3, pp. 307-15, (2004) (PubMed).

Puccetti, Bruni, Di Renzo, Bova, Cercignani, Iadanza, Auteri, Pasqui: "Hypercoagulable state in hypercholesterolemic subjects assessed by platelet-dependent thrombin generation: in vitro effect of cerivastatin." in: European review for medical and pharmacological sciences, Vol. 3, Issue 5, pp. 197-204, (2000) (PubMed).

Mennitt, Wade, Ecelbarger, Palmer, Frindt: "Localization of ROMK channels in the rat kidney." in: Journal of the American Society of Nephrology : JASN, Vol. 8, Issue 12, pp. 1823-30, (1998) (PubMed).

Target Details for Lipocalin 2

Target: Lipocalin 2 (LCN2)

Alternative Name: LCN2 (LCN2 Products)

Synonyms: LCN2 antibody, 24p3 antibody, MSFI antibody, NGAL antibody, AW212229 antibody, Sip24 antibody, lipocalin 2 antibody, LCN2 antibody, Lcn2 antibody

Background:

Background: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.

Aliases: NGAL,25 kDa alpha-2-microglobulin-related subunit of MMP-9,Lipocalin-2

UniProt: P80188

Pathways: Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis