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Antibodies
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anti-Neurofilament, Heavy Polypeptide 200kDa (NEFH) (200 kD) antibody
Neurofilament, Heavy Polypeptide 200kDa (NEFH) (200 kD) antibody
| Antigen |
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| Synonyms |
NFH, NEFH, nfh |
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Epitope
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| Clonality |
Monoclonal (RT97) |
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Host
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Reactivity
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Application
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| Catalog no. |
ABIN104615 |
| Quantity |
1ml (100ug/ml approximately) (Variants) |
| Price |
327.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Neurofilament H (200 kD)
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Immunogen
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Triton x 100 insoluble rat brain protein.
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Format
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Supernatant
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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RT97
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Specificity
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This antibody stains strongly with human neurofilaments of 200 kD. It stains neuromas, gangliomas, neuroblastomas and medulloblastomas. It also stains neuronal processes in brain and other nervous tissues. This antibody is useful to detect paragangliomas, phaeochromocytomas and other tumors of neuroectodermal origin.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Brain. Cellular localization: Cytoplasmic
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Cytoskeleton, Cell/Tissue Markers
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Publications
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Anderton, Breinburg, Downes et al.: "Monoclonal antibodies show that neurofibrillary tangles and neurofilaments share antigenic determinants." in: Nature, Vol. 298, Issue 5869, pp. 84-6, 1982 (PubMed).
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Alternatives for antigen "Neurofilament, Heavy Polypeptide 200kDa (NEFH)", type "Antibodies"
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Hosts
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Mouse (15), Rabbit (5), Chicken (4)
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Reactivities
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Human (19), Rat (Rattus) (14), Mouse (Murine) (10), Chicken (7), Cow (Bovine) (6), Pig (Porcine) (5), Reptile (3), Mammalian (2), Guinea Pig (1), Primate (1)
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Applications
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Immunohistochemistry (Frozen Sections) (IHC (fro)) (16), Western Blotting (WB) (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Immunofluorescence (IF) (10), Enzyme Immunoassay (EIA) (4), ELISA (1), ELISA (Capture) (1), Immunohistochemistry (IHC) (1)
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Epitopes
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200 kD (23)
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Alternatives 
