Neurofilament L&H (70/200 KD) antibody

Antigen: Neurofilament L&H (70/200 KD)

Clonality: Monoclonal (2F11)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN104617

Additional Information

Immunogen: BALB/C mice were injected with purified neurofilaments from human brain.

Format: Supernatant (prediluted)

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: 2F11

Specificity: This antibody reacts with 70 and 200 kD component of the polypeptide subunit. It stains neurons, neuronal processes and peripheral nerves. This antibody reacts with some neuroblastomas and gangliomas but not with other non-neuronal tumors.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Brain. Cellular localization: Cytoplasmic

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cytoskeleton, Cell/Tissue Markers

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Osborn, Dirk, Kaeser et al.: "Immunohistochemical localization of neurofilaments and neuron-specific enolase in 29 cases of neuroblastoma." in: The American journal of pathology, Vol. 122, Issue 3, pp. 433-42, 1986 (PubMed).

van Muijen, Ruiter, Warnaar: "Intermediate filaments in Merkel cell tumors." in: Human pathology, Vol. 16, Issue 6, pp. 590-5, 1985 (PubMed).

Marton, Feletti, Basaldella et al.: "Atypical cystic meningioma overexpressing AQP1 in early infancy: case report with literature review." in: Acta paediatrica (Oslo, Norway : 1992), Vol. 97, Issue 8, pp. 1145-9, 2008 (PubMed).