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ADAMTS5 antibody (Internal Region)

ADAMTS5 Reactivity: Human, Monkey IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1048294
  • Target See all ADAMTS5 Antibodies
    ADAMTS5 (ADAM Metallopeptidase with thrombospondin Type 1 Motif, 5 (ADAMTS5))
    Binding Specificity
    • 15
    • 8
    • 8
    • 7
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 55
    • 27
    • 20
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Monkey
    Host
    • 53
    • 3
    • 2
    Rabbit
    Clonality
    • 55
    • 3
    Polyclonal
    Conjugate
    • 26
    • 5
    • 5
    • 5
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ADAMTS5 antibody is un-conjugated
    Application
    • 48
    • 29
    • 10
    • 9
    • 7
    • 2
    • 2
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human ADAMTS5. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Predicted Reactivity
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Rat, Dog, Bat, Bovine, Hamster, Elephant, Panda, Rabbit, Pig (94%) Mouse, Horse, Turkey, Lizard, Xenopus (88%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic 16 amino acid peptide from internal region of human ADAMTS5. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Rat, Dog, Bat, Bovine, Hamster, Elephant, Panda, Rabbit, Pig (94%), Mouse, Horse, Turkey, Lizard, Xenopus (88%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product ADAMTS5 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (20 μg/mL)
    Comment

    Target Species of Antibody: Human

    Assay Procedure

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    ADAMTS5 (ADAM Metallopeptidase with thrombospondin Type 1 Motif, 5 (ADAMTS5))
    Alternative Name
    ADAMTS5 (ADAMTS5 Products)
    Synonyms
    9530092O11Rik antibody, ADAM-TS5 antibody, ADAMTS1 antibody, ADAMTS11 antibody, AI481094 antibody, ASMP-2 antibody, ADAM-TS 11 antibody, ADAM-TS 5 antibody, ADAMTS-11 antibody, ADAMTS-5 antibody, ADMP-2 antibody, ADAM metallopeptidase with thrombospondin type 1 motif 5 antibody, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2) antibody, ADAM metallopeptidase with thrombospondin type 1 motif, 5 antibody, ADAMTS5 antibody, adamts5 antibody, Adamts5 antibody
    Background
    Name/Gene ID: ADAMTS5
    Subfamily: Metallopeptidase M12B
    Family: Protease

    Synonyms: ADAMTS5, Aggrecanase-2, ADAM-TS 11, ADAM-TS5, ADAMTS-11, ADAMTS11, ADAM-TS 5, ADAMTS-5, ADMP-2, ADMP2
    Gene ID
    11096
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