Apelin Receptor antibody (Extracellular Domain)

Catalog No. ABIN1048324

Product Details anti-Apelin Receptor Antibody

Target: Apelin Receptor (APLNR)

Binding Specificity: Extracellular Domain

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Apelin Receptor antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human Apelin Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%) Horse, Rabbit (93%) Panda (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 14 amino acid peptide from 2nd extracellular domain of human Apelin Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%), Horse, Rabbit (93%), Panda (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 - 20 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Apelin Receptor

Target: Apelin Receptor (APLNR)

Alternative Name: APLNR/ Apelin Receptor / APJ (APLNR Products)

Synonyms: AGTRL1 antibody, APJ antibody, APJR antibody, HG11 antibody, Agtrl1 antibody, msr/apj antibody, Apj antibody, X-msr antibody, Xangio1 antibody, Xmsr antibody, agtrl1 antibody, angio1 antibody, apj antibody, apjr antibody, hg11 antibody, msr antibody, APLNR antibody, DKFZp469C1532 antibody, agtrl1-B antibody, agtrl1b antibody, Agtrl1a antibody, grn antibody, zgc:114063 antibody, APJa antibody, XAngio1 antibody, agtrl1-A antibody, agtrl1a antibody, aplnr-a antibody, aplnr-b antibody, x-MSR antibody, xAngio1 antibody, APLNR1 antibody, apelin receptor antibody, apelin receptor S homeolog antibody, apelin receptor b antibody, apelin receptor L homeolog antibody, APLNR antibody, Aplnr antibody, aplnr antibody, aplnr.S antibody, aplnrb antibody, aplnr.L antibody

Background: Name/Gene ID: APLNR
Subfamily: Apelin
Family: GPCR

Synonyms: APLNR, AGTRL1, Angiotensin receptor-like 1, Angiotensin II receptor-like 1, Apelin receptor, APJ, APJ (apelin) receptor, APJR, G protein-coupled receptor APJ, HG11 orphan receptor, HG11, APJ receptor, G-protein coupled receptor APJ

Gene ID: 187