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CCRL1 antibody (Cytoplasmic Domain)

CCRL1 Reactivity: Human IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1048409
  • Target See all CCRL1 Antibodies
    CCRL1 (Chemokine (C-C Motif) Receptor-Like 1 (CCRL1))
    Binding Specificity
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain
    Reactivity
    • 17
    • 10
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 16
    • 1
    Rabbit
    Clonality
    • 17
    Polyclonal
    Conjugate
    • 17
    This CCRL1 antibody is un-conjugated
    Application
    • 13
    • 9
    • 8
    • 4
    • 3
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human CCRL1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Predicted Reactivity
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%) Monkey, Rat, Bovine (84%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic 19 amino acid peptide from 2nd cytoplasmic domain of human CCRL1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%), Monkey, Rat, Bovine (84%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product CCRL1 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (24 - 32 μg/mL)
    Comment

    Target Species of Antibody: Human

    Assay Procedure

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    CCRL1 (Chemokine (C-C Motif) Receptor-Like 1 (CCRL1))
    Alternative Name
    ACKR4 / CCRL1 / CCR11 (CCRL1 Products)
    Synonyms
    CC-CKR-11 antibody, CCBP2 antibody, CCR-11 antibody, CCR10 antibody, CCR11 antibody, CCRL1 antibody, CCX CKR antibody, CCX-CKR antibody, CKR-11 antibody, PPR1 antibody, VSHK1 antibody, A630091E18Rik antibody, CCX-CKR1 antibody, Ccrl1 antibody, Cmkbrl1 antibody, atypical chemokine receptor 4 antibody, chemokine (C-C motif) receptor-like 1 antibody, Chemokine receptor-like 1 antibody, chemerin chemokine-like receptor 1 antibody, ACKR4 antibody, Ackr4 antibody, CCRL1 antibody, cml1 antibody, CMKLR1 antibody
    Background
    Name/Gene ID: ACKR4
    Subfamily: Chemokine
    Family: GPCR

    Synonyms: ACKR4, C-C CKR-11, CC-CKR-11, CC chemokine receptor-like 1, CCR11, CCX CKR, C-C chemokine receptor type 11, CKR-11, CCRL1, VSHK1, CCR-11, CCX-CKR, PPR1
    Gene ID
    51554
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