LPAR1 antibody (N-Term)

Catalog No. ABIN1048529

Product Details anti-LPAR1 Antibody

Target: LPAR1 (Lysophosphatidic Acid Receptor 1 (LPAR1))

Binding Specificity: N-Term

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LPAR1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human EDG2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except DDX60 (53 % ), ACER3 (47 %).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant (100%) Mouse, Rat, Sheep, Dog, Bovine, Bat, Hamster, Panda, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (93%) Xenopus, Pufferfish, Zebrafish (87%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 15 amino acid peptide from N-terminal extracellular domain of human EDG2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant (100%), Mouse, Rat, Sheep, Dog, Bovine, Bat, Hamster, Panda, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (93%), Xenopus, Pufferfish, Zebrafish (87%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (1 - 2 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for LPAR1

Target: LPAR1 (Lysophosphatidic Acid Receptor 1 (LPAR1))

Alternative Name: LPAR1 / LPA1 / EDG2 (LPAR1 Products)

Synonyms: EDG2 antibody, GPR26 antibody, Gpcr26 antibody, LPA1 antibody, Mrec1.3 antibody, VZG1 antibody, edg-2 antibody, rec.1.3 antibody, vzg-1 antibody, Edg2 antibody, EDG-2 antibody, 5031439C20 antibody, AI326300 antibody, Kdt2 antibody, lpA1 antibody, edg2 antibody, si:dz22b13.1 antibody, si:dz46m7.1 antibody, zgc:92157 antibody, LPA receptor 1-A antibody, LPA-1-A antibody, gpr26 antibody, lpa1 antibody, lpa1r antibody, lpa1r1 antibody, lpar1 antibody, lpar1-a antibody, lpar1-b antibody, vzg1 antibody, xLPA1-1 antibody, LPA receptor 1-B antibody, LPA-1-B antibody, edg2-A antibody, xLPA1-2 antibody, xlpa1R antibody, lysophosphatidic acid receptor 1 antibody, lysophosphatidic acid receptor 1 S homeolog antibody, lysophosphatidic acid receptor 1 L homeolog antibody, LPAR1 antibody, Lpar1 antibody, lpar1 antibody, lpar1.S antibody, lpar1.L antibody

Background: Name/Gene ID: LPAR1
Subfamily: Lysophospholipid/Lysosphingolipid
Family: GPCR

Synonyms: LPAR1, EDG-2, EDG2, Gpcr26, Rec.1.3, Vzg-1, Ventricular zone gene 1, LPA receptor 1, LPA-1, LPA1, Mrec1.3, VZG1

Gene ID: 1902

Pathways: Myometrial Relaxation and Contraction, Smooth Muscle Cell Migration