S1PR3 antibody (C-Term)

Catalog No. ABIN1048531

Product Details anti-S1PR3 Antibody

Target: S1PR3 (Sphingosine-1-Phosphate Receptor 3 (S1PR3))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S1PR3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human EDG3 / S1P3. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except TPRX1 (56 % ).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%) Marmoset, Elephant (94%) Bovine (89%) Gibbon, Rabbit (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from C-terminus of human EDG3 / S1P3. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%), Marmoset, Elephant (94%), Bovine (89%), Gibbon, Rabbit (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (4 - 20 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for S1PR3

Target: S1PR3 (Sphingosine-1-Phosphate Receptor 3 (S1PR3))

Alternative Name: SIPR3 / EDG3 / S1P3 (S1PR3 Products)

Synonyms: s1pr3 antibody, si:dkeyp-31e2.1 antibody, EDG-3 antibody, EDG3 antibody, LPB3 antibody, S1P3 antibody, AI132464 antibody, Edg3 antibody, Lpb3 antibody, S1p3 antibody, sphingosine-1-phosphate receptor 3 antibody, sphingosine-1-phosphate receptor 3a antibody, S1PR3 antibody, s1pr3a antibody, S1pr3 antibody

Background: Name/Gene ID: S1PR3
Subfamily: Lysophospholipid/Lysosphingolipid
Family: GPCR

Synonyms: S1PR3, EDG-3, EDG3, LPB3, S1P receptor EDG3, S1P3, S1P receptor Edg-3, S1P receptor 3

Gene ID: 1903