LPAR4 antibody (N-Term)

Catalog No. ABIN1049014

Product Details anti-LPAR4 Antibody

Target: LPAR4 (Lysophosphatidic Acid Receptor 4 (LPAR4))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Monkey, Hamster, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LPAR4 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Brand: IHC-plus™

Specificity: Human LPAR4 / GPR23. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bat, Hamster (100%) Dog, Bovine, Elephant, Panda, Rabbit, Horse, Pig (95%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 19 amino acid peptide from N-terminal extracellular domain of human LPAR4 / GPR23. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bat, Hamster (100%), Dog, Bovine, Elephant, Panda, Rabbit, Horse, Pig (95%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Flo, IHC, IHC-P (2 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

References for anti-LPAR4 antibody (ABIN1049014)

Lin, Boyce: "IL-4 regulates MEK expression required for lysophosphatidic acid-mediated chemokine generation by human mast cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 8, pp. 5430-8, (2005) (PubMed).

Target Details for LPAR4

Target: LPAR4 (Lysophosphatidic Acid Receptor 4 (LPAR4))

Alternative Name: LPAR4 / GPR23 (LPAR4 Products)

Synonyms: GPR23 antibody, gpr23 antibody, lpa4 antibody, LPA4 antibody, P2RY9 antibody, P2Y5-LIKE antibody, P2Y9 antibody, 5730485F04Rik antibody, Gpr23 antibody, p2y9 antibody, RGD1563686 antibody, lysophosphatidic acid receptor 4 antibody, lysophosphatidic acid receptor 4 L homeolog antibody, LPAR4 antibody, lpar4.L antibody, lpar4 antibody, Lpar4 antibody

Background: Name/Gene ID: LPAR4
Subfamily: Lysophospholipid/Lysosphingolipid
Family: GPCR

Synonyms: LPAR4, G-protein coupled receptor 23, LPA-4, p2RY9, p2Y5-LIKE, p2Y purinoceptor 9, p2Y5-like receptor, p2Y9, G protein-coupled receptor 23, GPR23, LPA receptor 4, LPA4, Purinergic receptor 9

Gene ID: 2846