MC3R antibody (C-Term)
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- Target See all MC3R Antibodies
- MC3R (Melanocortin 3 Receptor (MC3R))
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This MC3R antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human MC3 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except MC5R (78 % ).
- Predicted Reactivity
- Percent identity with other species by BLAST analysis: Human (100%) Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Pig (94%) Bat, Rabbit, Platypus (89%) Opossum, Turkey, Chicken, Xenopus, Trout (83%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic 18 amino acid peptide from C-Terminus of human MC3 Receptor. Percent identity with other species by BLAST analysis: Human (100%), Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Pig (94%), Bat, Rabbit, Platypus (89%), Opossum, Turkey, Chicken, Xenopus, Trout (83%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product MC3R Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (10 - 12 μg/mL)
Usage: Immunohistochemistry with formalin-fixed paraffin-embedded tissues requires pretreatment with Proteinase K. - Comment
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Target Species of Antibody: Human
- Assay Procedure
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- MC3R (Melanocortin 3 Receptor (MC3R))
- Alternative Name
- MC3R / MC3 Receptor (MC3R Products)
- Synonyms
- zgc:194235 antibody, MC3-R antibody, BMIQ9 antibody, MC3 antibody, OB20 antibody, OQTL antibody, melanocortin 3 receptor antibody, mc3r antibody, MC3R antibody, Mc3r antibody
- Background
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Name/Gene ID: MC3R
Subfamily: Melanocortin
Family: GPCR
Synonyms: MC3R, BMIQ9, MC3 receptor, Melanocortin receptor 3, OB20, MC3, MC3-R, Melanocortin 3 receptor, OQTL - Gene ID
- 4159
- Pathways
- cAMP Metabolic Process
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