NEK9 antibody

Catalog No. ABIN1049094

Product Details anti-NEK9 Antibody

Target: NEK9 (NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9))

Reactivity: Human, Horse, Rabbit, Pig, Monkey, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK9 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human NEK9. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Hamster, Elephant, Panda, Horse, Rabbit, Pig (100%) Mouse, Rat, Dog, Bovine, Bat, Opossum (93%) Platypus (87%) Turkey, Chicken (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 15 amino acid peptide from Pro/Ser/Thr rich domain of human NEK9. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Hamster, Elephant, Panda, Horse, Rabbit, Pig (100%), Mouse, Rat, Dog, Bovine, Bat, Opossum (93%), Platypus (87%), Turkey, Chicken (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL)

Usage: Proteinase K treatment is required for antigen retrieval.

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NEK9

Target: NEK9 (NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9))

Alternative Name: NEK9 (NEK9 Products)

Synonyms: NERCC antibody, NERCC1 antibody, Nek8 antibody, NEK9 antibody, nercc antibody, nercc1 antibody, C130021H08Rik antibody, NIMA related kinase 9 antibody, NIMA-related kinase 9 antibody, NIMA related kinase 8 antibody, NIMA (never in mitosis gene a)-related expressed kinase 9 antibody, NIMA-related kinase 9 L homeolog antibody, NEK9 antibody, nek9 antibody, NEK8 antibody, Nek9 antibody, nek9.L antibody

Background: Name/Gene ID: NEK9
Subfamily: NEK
Family: Protein Kinase

Synonyms: NEK9, KIAA1995, NERCC, NERCC1, Nercc1 kinase, NIMA-related kinase 8, NIMA-related kinase Nek8, NIMA-related kinase 9, NimA-related protein kinase 9

Gene ID: 91754

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection