NR2E1 antibody (N-Term)
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- Target See all NR2E1 Antibodies
- NR2E1 (Nuclear Receptor Subfamily 2, Group E, Member 1 (NR2E1))
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Binding Specificity
- N-Term
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Reactivity
- Human, Rabbit, Cow, Dog, Horse, Pig, Monkey, Bat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NR2E1 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human NR2E1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Predicted Reactivity
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Shrew, Bat, Bovine, Dog, Elephant, Panda, Rabbit, Horse, Pig, Opossum, Armadillo (100%) Mouse, Rat, Hamster, Sparrow, Chicken, Platypus, Lizard, Xenopus, Medaka, Pufferfish, Zebrafish, Stickleback (93%).
- Purification
- Immunoaffinity purified
- Immunogen
- Synthetic 15 amino acid peptide from a N-terminal region of human NR2E1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Shrew, Bat, Bovine, Dog, Elephant, Panda, Rabbit, Horse, Pig, Opossum, Armadillo (100%), Mouse, Rat, Hamster, Sparrow, Chicken, Platypus, Lizard, Xenopus, Medaka, Pufferfish, Zebrafish, Stickleback (93%).
- Top Product
- Discover our top product NR2E1 Primary Antibody
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- Application Notes
- Approved: IHC, IHC-P (5.5 μg/mL)
- Comment
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Target Species of Antibody: Human
- Assay Procedure
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- NR2E1 (Nuclear Receptor Subfamily 2, Group E, Member 1 (NR2E1))
- Alternative Name
- NR2E1 / TLX (NR2E1 Products)
- Synonyms
- NR2E1 antibody, TLL antibody, TLX antibody, XTLL antibody, Mtl1 antibody, Mtll antibody, Tlx antibody, fierce antibody, frc antibody, tailless antibody, fc30e03 antibody, fc39f12 antibody, wu:fc30e03 antibody, wu:fc39f12 antibody, zgc:100991 antibody, Xtll antibody, nr2e1-A antibody, tlx antibody, nuclear receptor subfamily 2 group E member 1 antibody, nuclear receptor subfamily 2, group E, member 1 antibody, nuclear receptor subfamily 2 group E member 1 S homeolog antibody, NR2E1 antibody, Nr2e1 antibody, nr2e1 antibody, nr2e1.S antibody
- Background
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Name/Gene ID: NR2E1
Subfamily: NR2 Hepatocyte NF4-like
Family: NHR
Synonyms: NR2E1, HTll, Tailless homolog, Tailless, TLX, TLL, Nuclear receptor TLX, Protein tailless homolog, Tailes-related receptor, XTLL - Gene ID
- 7101
- Pathways
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance
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