Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

PTGER4 antibody (C-Term)

PTGER4 Reactivity: Human, Mouse, Rat, Monkey, Dog, Cow, Bat, Hamster, Horse IHC (p), IHC, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1049258
  • Target See all PTGER4 Antibodies
    PTGER4 (Prostaglandin E Receptor 4 (Subtype EP4) (PTGER4))
    Binding Specificity
    • 16
    • 7
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    Human, Mouse, Rat, Monkey, Dog, Cow, Bat, Hamster, Horse
    Host
    • 62
    • 1
    • 1
    Rabbit
    Clonality
    • 64
    Polyclonal
    Conjugate
    • 31
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This PTGER4 antibody is un-conjugated
    Application
    • 50
    • 20
    • 14
    • 11
    • 7
    • 5
    • 4
    • 4
    • 3
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
    Brand
    IHC-plus™
    Specificity
    Human PTGER4 / EP4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Predicted Reactivity
    Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Dog, Bovine, Horse (100%) Turkey, Chicken (94%) Rabbit, Opossum, Guinea pig (89%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic 18 amino acid peptide from C-terminus of human PTGER4 / EP4. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Dog, Bovine, Horse (100%), Turkey, Chicken (94%), Rabbit, Opossum, Guinea pig (89%).

    Type of Immunogen: Synthetic peptide
  • Application Notes
    Approved: ICC, IHC, IHC-P (3.6 μg/mL)
    Comment

    Target Species of Antibody: Human

    Assay Procedure

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    PTGER4 (Prostaglandin E Receptor 4 (Subtype EP4) (PTGER4))
    Alternative Name
    PTGER4 / EP4 (PTGER4 Products)
    Synonyms
    EP4 antibody, PGE2R-EP4 antibody, ptger4 antibody, ptger4l antibody, PTGER4 antibody, EP4R antibody, Ptgerep4 antibody, Ptger antibody, ep4 antibody, prostaglandin E receptor 4 antibody, prostaglandin E receptor 4 (subtype EP4) antibody, prostaglandin E receptor 4 (subtype EP4) a antibody, prostaglandin E receptor 4 subtype EP4 antibody, PTGER4 antibody, ptger4a antibody, ptger4 antibody, Ptger4 antibody
    Background
    Name/Gene ID: PTGER4
    Subfamily: Prostanoid
    Family: GPCR

    Synonyms: PTGER4, EP4, EP4 prostaglandin receptor, EP4R, PGE receptor EP4 subtype, Prostanoid EP4 receptor, Prostaglandin E receptor 4, PGE receptor, EP4 subtype, PGE2 receptor EP4 subtype
    Gene ID
    5734
You are here:
Support