PTPRA antibody (Cytoplasmic Domain)

Catalog No. ABIN1049271

Product Details anti-PTPRA Antibody

Target: PTPRA (Protein tyrosine Phosphatase, Receptor Type, A (PTPRA))

Binding Specificity: Cytoplasmic Domain

Reactivity: Human, Mouse, Rat, Dog, Chicken, Cow, Pig, Monkey, Rabbit, Zebrafish (Danio rerio), Horse, Bat, Hamster, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTPRA antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human PTPRA. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except PTPRE (67 % ).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Seabass, Stickleback, Medaka, Pufferfish, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from cytoplasmic domain of human PTPRA. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Seabass, Stickleback, Medaka, Pufferfish, Zebrafish (100%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (4 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PTPRA

Target: PTPRA (Protein tyrosine Phosphatase, Receptor Type, A (PTPRA))

Alternative Name: PTPRA / RPTP-alpha (PTPRA Products)

Synonyms: PTPRA antibody, heptp antibody, hlpr antibody, hptpa antibody, hptpalpha antibody, lrp antibody, ptpa antibody, ptprl2 antibody, r-ptp-alpha antibody, rptpa antibody, HEPTP antibody, HLPR antibody, HPTPA antibody, HPTPalpha antibody, LRP antibody, PTPA antibody, PTPRL2 antibody, R-PTP-alpha antibody, RPTPA antibody, Ptpa antibody, Ptpalpha antibody, Rptpalpha antibody, Rptra antibody, Rptralpha antibody, RPTP[a] antibody, zf-RPTP[a] antibody, ptpa-a antibody, protein tyrosine phosphatase, receptor type A antibody, protein tyrosine phosphatase, receptor type, A antibody, protein tyrosine phosphatase, receptor type A S homeolog antibody, PTPRA antibody, ptpra antibody, Ptpra antibody, ptpra.S antibody

Background: Name/Gene ID: PTPRA
Subfamily: R4
Family: Protein Phosphatase

Synonyms: PTPRA, HLPR, Hptp alpha, HEPTP, HPTPA, PTPA, Ptpalpha, PTPRL2, PTP alpha, PTPLCA-related phosphatase, R-PTP-alpha, RPTPA, Rptpalpha, Tyrosine phosphatase alpha, RPTP alpha, HPTPalpha, LRP, PTPase-alpha

Gene ID: 5786