SCN1A antibody (N-Term)

Catalog No. ABIN1049319

Product Details anti-SCN1A Antibody

Target: SCN1A (Sodium Channel, Voltage-Gated, Type I, alpha Subunit (SCN1A))

Binding Specificity: N-Term

Reactivity: Human, Rat, Mouse, Chicken, Dog, Hamster, Horse, Monkey, Pig, Rabbit, Xenopus laevis, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCN1A antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human SCN1A / Nav1.1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except SCN2A (100 % ), SCN3A (94 %).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Xenopus, Pufferfish (100%) Bovine, Opossum, Stickleback, Zebrafish (94%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from N-terminus of human SCN1A / Nav1.1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Xenopus, Pufferfish (100%), Bovine, Opossum, Stickleback, Zebrafish (94%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SCN1A

Target: SCN1A (Sodium Channel, Voltage-Gated, Type I, alpha Subunit (SCN1A))

Alternative Name: SCN1A / Nav1.1 (SCN1A Products)

Synonyms: SCN9A antibody, SCN1A antibody, B230332M13 antibody, Nav1.1 antibody, EIEE6 antibody, FEB3 antibody, FEB3A antibody, FHM3 antibody, GEFSP2 antibody, HBSCI antibody, NAC1 antibody, SCN1 antibody, SMEI antibody, sodium voltage-gated channel alpha subunit 1 antibody, sodium voltage-gated channel alpha subunit 9 antibody, sodium channel, voltage-gated, type I, alpha antibody, SCN1A antibody, SCN9A antibody, Scn1a antibody

Background: Name/Gene ID: SCN1A
Subfamily: Sodium channel - voltage-gated
Family: Ion Channel

Synonyms: SCN1A, Brain sodium channel type I, FEB3A, FHM3, GEFSP2, EIEE6, HBSC I, HBSCI, GEFS+2, NAC1, Nav1.1, SCN1, SMEI, FEB3, Febrile convulsions 3, RBI

Gene ID: 6323