SLC11A2 antibody (Internal Region)
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- Target See all SLC11A2 Antibodies
- SLC11A2 (Solute Carrier Family 11 (Proton-Coupled Divalent Metal Ion Transporters), Member 2 (SLC11A2))
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Binding Specificity
- Internal Region
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Reactivity
- Human, Monkey
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SLC11A2 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human SLC11A2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Predicted Reactivity
- Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%) Galago, Rat, Hamster, Bovine, Horse (94%) Mouse, Elephant (88%) Dog, Bat, Rabbit, Pig, Guinea pig (81%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic 16 amino acid peptide from internal region of human SLC11A2. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%), Galago, Rat, Hamster, Bovine, Horse (94%), Mouse, Elephant (88%), Dog, Bat, Rabbit, Pig, Guinea pig (81%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product SLC11A2 Primary Antibody
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- Application Notes
- Approved: IHC, IHC-P (15 - 20 μg/mL)
- Comment
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Target Species of Antibody: Human
- Assay Procedure
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- SLC11A2 (Solute Carrier Family 11 (Proton-Coupled Divalent Metal Ion Transporters), Member 2 (SLC11A2))
- Alternative Name
- SLC11A2 / DMT1 (SLC11A2 Products)
- Synonyms
- DCT1 antibody, DMT1 antibody, NRAMP2 antibody, Nramp2 antibody, mk antibody, van antibody, Dmt1 antibody, ATNRAMP2 antibody, F8G22.4 antibody, F8G22_4 antibody, NRAMP metal ion transporter 2 antibody, dct1 antibody, dmt1 antibody, nramp2 antibody, SLC11A2 antibody, cb426 antibody, fa07b10 antibody, wu:fa07b10 antibody, zgc:136699 antibody, solute carrier family 11 member 2 antibody, solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2 antibody, NRAMP metal ion transporter 2 antibody, manganese transport protein antibody, metal transporter Nramp2 antibody, solute carrier family 11 (proton-coupled divalent metal ion transporter), member 2 antibody, SLC11A2 antibody, Slc11a2 antibody, NRAMP2 antibody, LOC9327403 antibody, slc11a2 antibody
- Background
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Name/Gene ID: SLC11A2
Subfamily: Natural resistance-associated macrophage protein (NRAMP)
Family: Transporter
Synonyms: SLC11A2, Divalent metal transporter 1, Divalent cation transporter 1, DMT-1, NRAMP 2, NRAMP2, Dct-1, DCT1, DMT1 - Gene ID
- 4891
- Pathways
- Transition Metal Ion Homeostasis, Proton Transport, Positive Regulation of Endopeptidase Activity
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