SLC7A2 antibody (Internal Region)

Catalog No. ABIN1049350

Product Details anti-SLC7A2 Antibody

Target: SLC7A2 (Solute Carrier Family 7 (Cationic Amino Acid Transporter, Y+ System), Member 2 (SLC7A2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC7A2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human SLC7A2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except TPR (67 % ).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Mouse, Rat, Rabbit, Pig (100%) Orangutan, Gibbon, Galago, Marmoset, Hamster, Bovine, Bat, Horse (92%) Panda, Dog, Platypus (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 12 amino acid peptide from internal region of human SLC7A2. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Mouse, Rat, Rabbit, Pig (100%), Orangutan, Gibbon, Galago, Marmoset, Hamster, Bovine, Bat, Horse (92%), Panda, Dog, Platypus (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (15 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 15 μg/mL.

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SLC7A2

Target: SLC7A2 (Solute Carrier Family 7 (Cationic Amino Acid Transporter, Y+ System), Member 2 (SLC7A2))

Alternative Name: SLC7A2 (SLC7A2 Products)

Synonyms: slc7a2 antibody, CAT2 antibody, CAT-2 antibody, MGC83504 antibody, zgc:103686 antibody, ATRC2 antibody, HCAT2 antibody, AI158848 antibody, Atrc2 antibody, Cat2 antibody, Tea antibody, Cat2a antibody, Cat2b antibody, RCAT2 antibody, cCAT-2 antibody, solute carrier family 7 (cationic amino acid transporter, y+ system), member 2, gene 2 L homeolog antibody, solute carrier family 7 (cationic amino acid transporter, y+ system), member 2 antibody, solute carrier family 7 member 2 antibody, slc7a2.2.L antibody, slc7a2 antibody, SLC7A2 antibody, Slc7a2 antibody

Background: Name/Gene ID: SLC7A2
Subfamily: Cationic amino acid transporter
Family: Transporter

Synonyms: SLC7A2, ATRC2, CAT2, HCAT2, CAT-2

Gene ID: 6542