Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) antibody

Antigen: Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1)

Synonyms: B23, NPM, MGC104254, Npm, NO38, MGC102162, MGC107291, B23NP, MGC108517, npm1, MGC53901, npm

Clonality: Monoclonal (NA24)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN104953

Additional Information

Alternative name: Nucleophosmin

Immunogen: BALB/C mice were immunized with GST fusion protein containing the N-terminal part of nucleophosmin fused to 14 amino acid of ALK protein.

Format: Purified

Isotype: IgG kappa  (Matching secondary antibodies)

Clone: NA24

Specificity: Nucleophosmin (NPM) / B23 / numatrin is a phosphoprotein associated with cell nucleoli. Treatment of cells with cytotoxic drugs may make nucleophosmin enter nucleoplasm. It is reported that nucleophosmin shuttles continuously between cytoplasm and nucleoli to carry newly synthesized ribosomal proteins into nucleoli. NPM-ALK is a hybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM) gene resulting from the t(2,5)(p23,q35) translocation found in anaplastic large cell lymphoma. In acute promyelocytic leukemia NPM gene is fused to RARalpha gene in (5,17) (q33,q12) transition

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified ascites containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Cordell, Pulford, Bigerna et al.: "Detection of normal and chimeric nucleophosmin in human cells." in: Blood, Vol. 93, Issue 2, pp. 632-42, 1999 (PubMed).