Estrogen Receptor (ER) antibody

Antigen: Estrogen Receptor (ER)

Synonyms: LOC398733, LOC398734, ERa, ESR, Estr, ER[a], Estra, Nr3a1, ERalpha, AA420328, AU041214, ER-alpha, ESR1, NR3A1, eralpha, zfER[a], Errra, ERRalpha, ESRRA, err1, erra, esrral, erralpha, LOC100125507, ESTRRA, esrl1, nr3b1, MGC147343, ERRa, ER[b]1, zfER[b]1, MGC103724, zfER-beta1, erb2, erb1, CERB, ESR2, ERB, erb, esrb, estrb, nr3a2, ERbeta, er-beta, esr-beta, ERBETA, er, ER-beta, ER[b], Estrb, ER[b]2, ER[b]a, zfER[b]2, zfER-beta2, LOC100125508, errb2, ER, Era, ESRA, DKFZp686N23123, 7B4, CD144, FLJ17376, ACTGP1, ACT1GP1, HY-psi-gamma-AC6, Esr, RNESTROR, ERALPHA, esr, esr1, nr3a1, AAC2, PNAT, XER, era, esra, xesr-1, xlERalpha1, xlERalpha2

Clonality: Monoclonal (6F11)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN105026

Additional Information

Alternative name: Oestrogen Receptor

Immunogen: Prokaryotic recombinant protein corresponding to the full-length estrogen receptor molecule.

Format: Supernatant (prediluted)

Isotype: IgG1 kappa

Clone: 6F11

Specificity: This antibody is specific to the estrogen receptor and recognizes full-length polypeptide chain of the receptor. The nuclei of the estrogen receptor positive cells stain very strongly with this antibody, without any staining in the cytoplasm.

Synonyms: LOC398733, LOC398734, ERa, ESR, Estr, ER[a], Estra, Nr3a1, ERalpha, AA420328, AU041214, ER-alpha, ESR1, NR3A1, eralpha, zfER[a], Errra, ERRalpha, ESRRA, err1, erra, esrral, erralpha, LOC100125507, ESTRRA, esrl1, nr3b1, MGC147343, ERRa, ER[b]1, zfER[b]1, MGC103724, zfER-beta1, erb2, erb1, CERB, ESR2, ERB, erb, esrb, estrb, nr3a2, ERbeta, er-beta, esr-beta, ERBETA, er, ER-beta, ER[b], Estrb, ER[b]2, ER[b]a, zfER[b]2, zfER-beta2, LOC100125508, errb2, ER, Era, ESRA, DKFZp686N23123, 7B4, CD144, FLJ17376, ACTGP1, ACT1GP1, HY-psi-gamma-AC6, Esr, RNESTROR, ERALPHA, esr, esr1, nr3a1, AAC2, PNAT, XER, era, esra, xesr-1, xlERalpha1, xlERalpha2

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Nuclear

Concentration: 1 to 4 ug/ml

Purification: Supernatant (prediluted)

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Hormones, Receptors

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Bur, Zimarowski, Schnitt et al.: "Estrogen receptor immunohistochemistry in carcinoma in situ of the breast." in: Cancer, Vol. 69, Issue 5, pp. 1174-81, 1992 (PubMed).

Graham, Jin, Lloyd: "Detection of estrogen receptor in paraffin-embedded sections of breast carcinoma by immunohistochemistry and in situ hybridization." in: The American journal of surgical pathology, Vol. 15, Issue 5, pp. 475-85, 1991 (PubMed).

Pertschuk, Kim, Nayer et al.: "Immunocytochemical estrogen and progestin receptor assays in breast cancer with monoclonal antibodies. Histopathologic, demographic, and biochemical correlations and relationship to endocrine response and survival." in: Cancer, Vol. 66, Issue 8, pp. 1663-70, 1990 (PubMed).

Kumar, Green, Stack et al.: "Functional domains of the human estrogen receptor." in: Cell, Vol. 51, Issue 6, pp. 941-51, 1988 (PubMed).

Ozono, Sato, Ito et al.: "Immunohistochemical evidence that tumors elicit the synthesis of estrogen receptors in the submandibular gland of female rats." in: Experientia, Vol. 51, Issue 3, pp. 220-2, 1995 (PubMed).