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Osteopontin antibody (Secreted phosphoprotein 1)

Details for Product anti-SPP1 Antibody No. ABIN105163, Supplier: Login to see New
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Synonyms zgc:111821, BNSP, BSPI, ETA-1, OPN, 2AR, Apl-1, Bsp, Eta, OP, Opn, Opnl, Ric, Spp-1, OSP, AI463453, SPP, SPP1, Spph1, SPPase1
(345), (81), (50), (21), (7), (3), (2), (1), (1)
(222), (153), (26), (4)
This Osteopontin antibody is un-conjugated
(22), (19), (18), (11), (9), (7), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(323), (228), (91), (74), (23), (22), (19), (14), (13), (11), (6), (4), (2), (2), (1), (1), (1), (1), (1)
Pubmed 18 references available
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Quantity 200 μL
Shipping to United States ( )
Availability Will be delivered in 2 to 3 Business Days
Immunogen This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, from the human osteopontin protein, conjugated to KLH using maleimide.
Characteristics Concentration Definition: by Refractometry
Purification Antiserum
Alternative Name Osteopontin (SPP1 Antibody Abstract)
Background Anti Osteopontin Antibody recognizes Osteopontin (OPN) that is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein which interacts with integrins and CD44 as major receptors. OPN is multifunctional, with activities in cell migration, cell survival, inhibition of calcification, regulation of immune cell function, and control of tumor cell phenotype. The gene encoding OPN is called spp1.  Targeting this gene has revealed that while OPN is not necessary for normal embryonic development, fertility and health under pathogen-free conditions, loss of the protein has significant consequences in several models of injury/disease as diverse as renal injury, viral and bacterial infection, bone remodeling, and tumor growth. The fact that no other proteins seem to share a redundant activity with OPN under these conditions suggests that OPN has a unique functional role during tissue injury and stress. Interestingly, several members of the matrix metalloproteinase (MMP) family are also induced during injury/disease processes in patterns overlapping that of OPN.  OPN has recently been shown to be a novel substrate for two MMPs, MMP-3 (stromelysin-1) and MMP-7 (matrilysin). There are three cleavage sites for MMP-3 in human OPN, two of which are also cleaved by MMP-7 (see cleavage diagram). Biological assays demonstrate that the MMP-cleaved OPN has increased activity in promoting both cell adhesion and migration compared with full-length OPN. In addition, inhibitory reagents were used to show that the same receptors that interact with OPN also mediate interaction of MMP-cleaved OPN with tumor cells.  It is suggested that active forms of OPN at sites of tissue injury may be regulated by the activity of proteases including MMPs and that the differences in activity of modified OPN may be explained by differences in binding affinity of integrins or distinct downstream signaling events.
Synonyms: Osteopontin Bone sialoprotein 1 Secreted phosphoprotein 1 SPP-1 Urinary stone protein Nephropontin Uropontin SPP1 BNSP, OPN ORF Name: PSEC0156
Gene ID 6696
UniProt P10451
Research Area Cancer, Extracellular Matrix, Hormones, Enzymes, Metabolism
Application Notes This antibody is suitable for western blotting, immunohistochemistry (formalin-fixed paraffin-embedded sections) and ELISA. The antibody exclusively recognizes C-terminal fragments of both thrombin and MMP-cleaved OPN.  A 1:1000 dilution will detect strongly approximately 250 ng of OPN protein on a blot. No pretreatment is required for IHC when formalin-fixed paraffin-embedded tissue is stained.
Restrictions For Research Use only
Format Liquid
Concentration 75 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Immunohistochemistry (IHC) image for anti-Osteopontin antibody (Secreted phosphoprotein 1) (ABIN105163) Rabbit anti-osteopontin was used at a 1:100-1:300 dilution to detect osteopontin by i...
Western Blotting (WB) image for anti-Osteopontin antibody (Secreted phosphoprotein 1) (ABIN105163) Osteopontin cleavage diagram. OPN is cleaved by MMP to yield 2 fragments, which migra...
 image for anti-Osteopontin antibody (Secreted phosphoprotein 1) (ABIN105163) anti-Secreted phosphoprotein 1 (SPP1) antibody (Image 3)
Product cited in: Sasaki, Matsumoto, Egusa et al.: "In vitro reproduction of endochondral ossification using a 3D mesenchymal stem cell construct." in: Integrative biology : quantitative biosciences from nano to macro, 2012 (PubMed).

Iwanaga, Ueno, Ueki et al.: "The expression of osteopontin is increased in vessels with blood-brain barrier impairment." in: Neuropathology and applied neurobiology, Vol. 34, Issue 2, pp. 145-54, 2008 (PubMed).

Kim, Shin: "Immunohistochemical study of osteopontin in boar testis." in: Journal of veterinary science, Vol. 8, Issue 2, pp. 107-10, 2007 (PubMed).

Shin, Ahn, Kim et al.: "Temporal expression of osteopontin and CD44 in rat brains with experimental cryolesions." in: Brain research, Vol. 1041, Issue 1, pp. 95-101, 2005 (PubMed).

Background publications Agnihotri, Crawford, Haro et al.: "Osteopontin, a novel substrate for matrix metalloproteinase-3 (stromelysin-1) and matrix metalloproteinase-7 (matrilysin)." in: The Journal of biological chemistry, Vol. 276, Issue 30, pp. 28261-7, 2001 (PubMed).

McCawley, Matrisian: "Matrix metalloproteinases: multifunctional contributors to tumor progression." in: Molecular medicine today, Vol. 6, Issue 4, pp. 149-56, 2000 (PubMed).

Ashkar, Weber, Panoutsakopoulou et al.: "Eta-1 (osteopontin): an early component of type-1 (cell-mediated) immunity." in: Science (New York, N.Y.), Vol. 287, Issue 5454, pp. 860-4, 2000 (PubMed).

Ophascharoensuk, Giachelli, Gordon et al.: "Obstructive uropathy in the mouse: role of osteopontin in interstitial fibrosis and apoptosis." in: Kidney international, Vol. 56, Issue 2, pp. 571-80, 1999 (PubMed).

Nau, Liaw, Chupp et al.: "Attenuated host resistance against Mycobacterium bovis BCG infection in mice lacking osteopontin." in: Infection and immunity, Vol. 67, Issue 8, pp. 4223-30, 1999 (PubMed).

Noiri, Dickman, Miller et al.: "Reduced tolerance to acute renal ischemia in mice with a targeted disruption of the osteopontin gene." in: Kidney international, Vol. 56, Issue 1, pp. 74-82, 1999 (PubMed).

Yoshitake, Rittling, Denhardt et al.: "Osteopontin-deficient mice are resistant to ovariectomy-induced bone resorption." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 14, pp. 8156-60, 1999 (PubMed).

Crawford, Matrisian, Liaw: "Distinct roles of osteopontin in host defense activity and tumor survival during squamous cell carcinoma progression in vivo." in: Cancer research, Vol. 58, Issue 22, pp. 5206-15, 1998 (PubMed).

Rittling, Matsumoto, McKee et al.: "Mice lacking osteopontin show normal development and bone structure but display altered osteoclast formation in vitro." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 13, Issue 7, pp. 1101-11, 1998 (PubMed).

Liaw, Birk, Ballas et al.: "Altered wound healing in mice lacking a functional osteopontin gene (spp1)." in: The Journal of clinical investigation, Vol. 101, Issue 7, pp. 1468-78, 1998 (PubMed).

Uede, Katagiri, Iizuka et al.: "Osteopontin, a coordinator of host defense system: a cytokine or an extracellular adhesive protein?" in: Microbiology and immunology, Vol. 41, Issue 9, pp. 641-8, 1997 (PubMed).

Weber, Cantor: "The immunology of Eta-1/osteopontin." in: Cytokine & growth factor reviews, Vol. 7, Issue 3, pp. 241-8, 1997 (PubMed).

Denhardt, Lopez, Rollo et al.: "Osteopontin-induced modifications of cellular functions." in: Annals of the New York Academy of Sciences, Vol. 760, pp. 127-42, 1995 (PubMed).

Denhardt, Chambers: "Overcoming obstacles to metastasis--defenses against host defenses: osteopontin (OPN) as a shield against attack by cytotoxic host cells." in: Journal of cellular biochemistry, Vol. 56, Issue 1, pp. 48-51, 1995 (PubMed).

Catalog No. ABIN105163
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