Cyclin-Dependent Kinase Inhibitor 2B (p15, Inhibits CDK4) (CDKN2B) antibody

Antigen: Cyclin-Dependent Kinase Inhibitor 2B (p15, Inhibits CDK4) (CDKN2B)

Synonyms: P15, MTS2, TP15, CDK4I, INK4B, p15INK4b, p15, INK4b, AV083695, p15(INK4b), CDKN2A, CDKN2B, mts2, tp15, ink4b, MGC89547, cdk4l, p15ink4b, MGC154405

Clonality: Monoclonal (15P06)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN105213

Additional Information

Alternative name: p15ink4b

Immunogen: BALB/C mice were immunized with purified human recombinant full length p15INK4b

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: 15P06

Specificity: This antibody with a protein of 15 kD. p15INK4b is a specific inhibitor of cdk4/cdk6 and is periodically expressed during cell cycle. p15INK4b expression is induced approximately 30fold in human keratinocytes by treatment with TGF-beta, suggesting that p15INK4b may act as an effector of TCF-beta mediated cell cycle arrest.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Colon carcinoma. Cellular localization: Nuclear Other Applications: Immunoprecipitation, Western blotting (1:100-500).

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified ascites containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human colon carcinoma stained with p15ink4b antibody

Publications

Publications: Jen, Harper, Bigner et al.: "Deletion of p16 and p15 genes in brain tumors." in: Cancer research, Vol. 54, Issue 24, pp. 6353-8, 1995 (PubMed).

Gray-Bablin, Rao, Keyomarsi: "Lovastatin induction of cyclin-dependent kinase inhibitors in human breast cells occurs in a cell cycle-independent fashion." in: Cancer research, Vol. 57, Issue 4, pp. 604-9, 1997 (PubMed).