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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Tumor Protein P53 (TP53) antibody
|Synonyms||p53, LFS1, TRP53, FLJ92943, P53, Trp53, MGC112612, brp53, drp53, fb40d06, wu:fb40d06, zgc:111919, TP53, bbl, bfy, bhy, p44, Tp53, tp53|
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|3 references available|
|Quantity||1ml (100ug/ml approximately) (Variants)|
|Price||454.30 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 3 to 4 Business Days|
|Immunogen||Recombinant wild type p53 protein.|
|Specificity||This antibody is specific to human p53 protein, both wild and mutant forms under denaturing and non-denaturing conditions. It maps the n-terminus region of the p53 molecule in the region of amino acids 20 and 25.|
|Application Notes||Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Nuclear|
|Buffer||This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.|
|Storage||Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.|
|Research Area||Transcription Factors|
|Restrictions||For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union|
Kern, Pietenpol, Thiagalingam et al.: "Oncogenic forms of p53 inhibit p53-regulated gene expression." in: Science (New York, N.Y.), Vol. 256, Issue 5058, pp. 827-30, 1992 (PubMed).
Malkin, Li, Strong et al.: "Germ line p53 mutations in a familial syndrome of breast cancer, sarcomas, and other neoplasms." in: Science (New York, N.Y.), Vol. 250, Issue 4985, pp. 1233-8, 1991 (PubMed).
Bártek, Bártková, Lukás et al.: "Immunohistochemical analysis of the p53 oncoprotein on paraffin sections using a series of novel monoclonal antibodies." in: The Journal of pathology, Vol. 169, Issue 1, pp. 27-34, 1993 (PubMed).