Tumor Protein P53 (TP53) antibody

Antigen: Tumor Protein P53 (TP53)

Synonyms: p53, LFS1, TRP53, FLJ92943, P53, Trp53, MGC112612, brp53, drp53, fb40d06, wu:fb40d06, zgc:111919, TP53, bbl, bfy, bhy, p44, Tp53, tp53

Clonality: Monoclonal (Bp53-12)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN105242

Additional Information

Alternative name: p53

Immunogen: Recombinant wild type p53 protein.

Format: Supernatant

Isotype: IgG2a

Clone: Bp53-12

Specificity: This antibody is specific to human p53 protein, both wild and mutant forms under denaturing and non-denaturing conditions. It maps the n-terminus region of the p53 molecule in the region of amino acids 20 and 25.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Purification: Supernatant

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Transcription Factors

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Kern, Pietenpol, Thiagalingam et al.: "Oncogenic forms of p53 inhibit p53-regulated gene expression." in: Science (New York, N.Y.), Vol. 256, Issue 5058, pp. 827-30, 1992 (PubMed).

Malkin, Li, Strong et al.: "Germ line p53 mutations in a familial syndrome of breast cancer, sarcomas, and other neoplasms." in: Science (New York, N.Y.), Vol. 250, Issue 4985, pp. 1233-8, 1991 (PubMed).

Bártek, Bártková, Lukás et al.: "Immunohistochemical analysis of the p53 oncoprotein on paraffin sections using a series of novel monoclonal antibodies." in: The Journal of pathology, Vol. 169, Issue 1, pp. 27-34, 1993 (PubMed).