Tumor Protein P53 (TP53) antibody

Antigen: Tumor Protein P53 (TP53)

Synonyms: p53, LFS1, TRP53, FLJ92943, P53, Trp53, MGC112612, brp53, drp53, fb40d06, wu:fb40d06, zgc:111919, TP53, bbl, bfy, bhy, p44, Tp53, tp53

Clonality: Monoclonal (Bp53-12)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN105243

Additional Information

Alternative name: p53

Immunogen: Recombinant human wild type p53 protein.

Format: Supernatant (prediluted)

Isotype: IgG2a kappa

Clone: Bp53-12

Specificity: This antibody reacts with an amino terminal epitope (residue 37-45) of human wild and mutant p53 and is non-reactive with murine p53. p53 has been classified as tumor suppressor gene or anti-oncogene. Presence of excess amounts of the mutant form of the protein can lead to functional inactivation of wild type p53. Mutations and allelic loss of the p53 gene have been associated with malignant transformation in a wide variety of human tumors.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Nuclear

Concentration: 1 to 4 ug/ml

Purification: Supernatant (prediluted)

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Transcription Factors

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Bártek, Bártková, Vojtĕsek et al.: "Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of human malignancies." in: Oncogene, Vol. 6, Issue 9, pp. 1699-703, 1991 (PubMed).

Malkin, Li, Strong et al.: "Germ line p53 mutations in a familial syndrome of breast cancer, sarcomas, and other neoplasms." in: Science (New York, N.Y.), Vol. 250, Issue 4985, pp. 1233-8, 1991 (PubMed).

Cattoretti, Rilke, Andreola et al.: "P53 expression in breast cancer." in: International journal of cancer. Journal international du cancer, Vol. 41, Issue 2, pp. 178-83, 1988 (PubMed).