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p65 antibody (Nuclear Factor-KB P65) (pSer529)

Details for Product anti-NFkBP65 Antibody No. ABIN105287, Supplier: Login to see
Antigen
  • NFKB3
  • p65
  • NFkB
Alternatives
anti-Human p65 antibody for Western Blotting
Epitope
pSer529
106
80
69
53
50
47
44
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42
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34
26
22
16
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2
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Reactivity
Human
1049
473
390
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37
26
12
10
6
4
4
3
2
2
2
1
1
1
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1
Host
Rabbit
974
81
13
8
2
Clonality
Polyclonal
Conjugate
This p65 antibody is un-conjugated
31
30
22
19
16
16
11
9
9
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8
1
1
1
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1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
769
407
335
172
150
133
90
42
30
24
21
20
10
9
7
7
7
4
2
2
1
1
Supplier
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Immunogen NFkB p65 (Rel A) peptide corresponding to a region near phospho Serine 529 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Characteristics Concentration Definition: by Refractometry
Purification Antiserum
Alternative Name NFkB p65 (RelA) (NFkBP65 Antibody Abstract)
Background NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. Anti-NFkB antibody is ideal for Cell Biology, Nuclear Signaling, Neuroscience and Signal Transduction Research
Synonyms: NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB
Gene ID 5970, 223468676
UniProt Q04206
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Anti-phospho NFkB antibody reacts human pS529 p65 and shows minimal reactivity by western blot with non-phosphorylated p65 and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.  Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.
Restrictions For Research Use only
Format Liquid
Concentration 75 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Western Blotting (WB) image for anti-p65 antibody (Nuclear Factor-KB P65) (pSer529) (ABIN105287) TNF Induces phosphorylation of p65 in KBM-5 cells.  Cytoplasmic and nuclear protein l...
Western Blotting (WB) image for anti-p65 antibody (Nuclear Factor-KB P65) (pSer529) (ABIN105287) anti-Nuclear Factor-KB P65 (NFkBP65) (pSer529) antibody (Image 2)
 image for anti-p65 antibody (Nuclear Factor-KB P65) (pSer529) (ABIN105287) anti-Nuclear Factor-KB P65 (NFkBP65) (pSer529) antibody (Image 3)
 image for anti-p65 antibody (Nuclear Factor-KB P65) (pSer529) (ABIN105287) anti-Nuclear Factor-KB P65 (NFkBP65) (pSer529) antibody (Image 4)
Product cited in: Gavert, Ben-Shmuel, Lemmon et al.: "Nuclear factor-kappaB signaling and ezrin are essential for L1-mediated metastasis of colon cancer cells." in: Journal of cell science, Vol. 123, Issue Pt 12, pp. 2135-43, 2010 (PubMed).

Amith, Jayanth, Franchuk et al.: "Neu1 desialylation of sialyl alpha-2,3-linked beta-galactosyl residues of TOLL-like receptor 4 is essential for receptor activation and cellular signaling." in: Cellular signalling, 2009 (PubMed).

Shakibaei, John, Schulze-Tanzil et al.: "Suppression of NF-kappaB activation by curcumin leads to inhibition of expression of cyclo-oxygenase-2 and matrix metalloproteinase-9 in human articular chondrocytes: Implications for the treatment of osteoarthritis." in: Biochemical pharmacology, Vol. 73, Issue 9, pp. 1434-45, 2007 (PubMed).

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