Nuclear Factor-KB P65 (NFkBP65) (pSer529) antibody

Details for Product No. ABIN105287
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Antigen
Synonyms c-Rel, C-Rel, Xrel2, c-rel, rel-A, v-rel, xrel, NFKB3, p65, NFkB, zgc:100833
Epitope
pSer529
(66), (57), (46), (45), (36), (34), (33), (31), (31), (30), (28), (20), (19), (12), (11), (7), (7), (7), (7), (6), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(854), (445), (354), (76), (75), (74), (38), (9), (8), (6), (2), (2), (1), (1)
Host
Rabbit
(799), (67), (6), (5), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(24), (19), (15), (11), (10), (10), (10), (10), (10), (10), (10), (10), (10), (10), (1), (1), (1)
Application
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
(655), (335), (331), (170), (129), (122), (100), (38), (21), (18), (16), (12), (7), (5), (2), (1)
Pubmed 3 references available
Quantity 100 μL
Options
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Catalog No. ABIN105287
361.90 $
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Immunogen NFkB p65 (Rel A) peptide corresponding to a region near phospho Serine 529 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Characteristics Concentration Definition: by Refractometry
Purification Antiserum
Alternative Name NFkB p65 (RelA)
Background NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. Anti-NFkB antibody is ideal for Cell Biology, Nuclear Signaling, Neuroscience and Signal Transduction Research
Synonyms: NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB
Gene ID 5970, 223468676
UniProt Q04206
Application Notes Anti-phospho NFkB antibody reacts human pS529 p65 and shows minimal reactivity by western blot with non-phosphorylated p65 and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.  Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.
Restrictions For Research Use only
Format Liquid
Concentration 75 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Nuclear Factor-KB P65 (NFkBP65) (pSer529) antibody TNF Induces phosphorylation of p65 in KBM-5 cells.  Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65.  Immunochemical pan reactive anti p65 (code# 100-4165) was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts.  Phosphorylation of p65 occurs after approximately 10 min of TNF exposure.  Migration of phosphorylated p65 into the nucleus occurs within a similar time frame.   HRP conjugated Gt-anti-Rabbit IgG was used to develop the western blot using a chemi-luminescent detection method.  Other detection methods will yield similar results.
anti-Nuclear Factor-KB P65 (NFkBP65) (pSer529) antibody (2) anti-Nuclear Factor-KB P65 (NFkBP65) (pSer529) antibody (Image 2)
anti-Nuclear Factor-KB P65 (NFkBP65) (pSer529) antibody (3) anti-Nuclear Factor-KB P65 (NFkBP65) (pSer529) antibody (Image 3)
anti-Nuclear Factor-KB P65 (NFkBP65) (pSer529) antibody (4) anti-Nuclear Factor-KB P65 (NFkBP65) (pSer529) antibody (Image 4)
General Shakibaei, John, Schulze-Tanzil et al.: "Suppression of NF-kappaB activation by curcumin leads to inhibition of expression of cyclo-oxygenase-2 and matrix metalloproteinase-9 in human articular chondrocytes: Implications for the treatment of osteoarthritis." in: Biochemical pharmacology, Vol. 73, Issue 9, pp. 1434-45, 2007 (PubMed).

Amith, Jayanth, Franchuk et al.: "Neu1 desialylation of sialyl alpha-2,3-linked beta-galactosyl residues of TOLL-like receptor 4 is essential for receptor activation and cellular signaling." in: Cellular signalling, 2009 (PubMed).

Gavert, Ben-Shmuel, Lemmon et al.: "Nuclear factor-kappaB signaling and ezrin are essential for L1-mediated metastasis of colon cancer cells." in: Journal of cell science, Vol. 123, Issue Pt 12, pp. 2135-43, 2010 (PubMed).

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