anti-p65 antibody (Nuclear Factor-KB P65) (pSer529)

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Antigen
Synonyms NFKB3, p65, NFkB
Alternatives
Epitope
pSer529
(107), (82), (72), (54), (50), (48), (46), (45), (43), (41), (36), (26), (17), (17), (16), (15), (13), (13), (13), (12), (10), (10), (10), (9), (9), (9), (7), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(1127), (531), (437), (40), (34), (23), (14), (10), (6), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(1049), (85), (13), (8), (2)
Clonality
Polyclonal
Conjugate
This p65 antibody is un-conjugated
(31), (30), (22), (19), (16), (16), (11), (9), (9), (8), (8), (8), (8), (8), (8), (2), (2), (1), (1), (1), (1)
Application
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
(833), (457), (384), (187), (171), (145), (90), (43), (30), (28), (21), (21), (11), (10), (10), (7), (6), (4), (2), (2), (2), (1)
Pubmed 3 references available
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Quantity 100 μL
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Immunogen NFkB p65 (Rel A) peptide corresponding to a region near phospho Serine 529 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Characteristics Concentration Definition: by Refractometry
Purification Antiserum
Alternative Name NFkB p65 (RelA) (NFkBP65 Antibody Abstract)
Background NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. Anti-NFkB antibody is ideal for Cell Biology, Nuclear Signaling, Neuroscience and Signal Transduction Research
Synonyms: NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB
Gene ID 5970, 223468676
UniProt Q04206
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Anti-phospho NFkB antibody reacts human pS529 p65 and shows minimal reactivity by western blot with non-phosphorylated p65 and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.  Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.
Restrictions For Research Use only
Format Liquid
Concentration 75 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Western Blotting (WB) image for anti-p65 antibody (Nuclear Factor-KB P65) (pSer529) (ABIN105287) TNF Induces phosphorylation of p65 in KBM-5 cells.  Cytoplasmic and nuclear protein l...
Western Blotting (WB) image for anti-p65 antibody (Nuclear Factor-KB P65) (pSer529) (ABIN105287) anti-Nuclear Factor-KB P65 (NFkBP65) (pSer529) antibody (Image 2)
Image no. 3 for anti-p65 antibody (Nuclear Factor-KB P65) (pSer529) (ABIN105287) anti-Nuclear Factor-KB P65 (NFkBP65) (pSer529) antibody (Image 3)
Image no. 4 for anti-p65 antibody (Nuclear Factor-KB P65) (pSer529) (ABIN105287) anti-Nuclear Factor-KB P65 (NFkBP65) (pSer529) antibody (Image 4)
Product cited in: Gavert, Ben-Shmuel, Lemmon et al.: "Nuclear factor-kappaB signaling and ezrin are essential for L1-mediated metastasis of colon cancer cells." in: Journal of cell science, Vol. 123, Issue Pt 12, pp. 2135-43, 2010 (PubMed).

Amith, Jayanth, Franchuk et al.: "Neu1 desialylation of sialyl alpha-2,3-linked beta-galactosyl residues of TOLL-like receptor 4 is essential for receptor activation and cellular signaling." in: Cellular signalling, 2009 (PubMed).

Shakibaei, John, Schulze-Tanzil et al.: "Suppression of NF-kappaB activation by curcumin leads to inhibition of expression of cyclo-oxygenase-2 and matrix metalloproteinase-9 in human articular chondrocytes: Implications for the treatment of osteoarthritis." in: Biochemical pharmacology, Vol. 73, Issue 9, pp. 1434-45, 2007 (PubMed).

Catalog No. ABIN105287
$405.90
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