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Antibodies
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anti-Retinoblastoma-Like 2 (p130) (RBL2) antibody
Retinoblastoma-Like 2 (p130) (RBL2) antibody
| Antigen |
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| Synonyms |
RBL2, MGC154964 |
| Clonality |
Monoclonal (130P215) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. |
ABIN105305 |
| Quantity |
1ml (100ug/ml approximately) (Variants) |
| Price |
481.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Discontinued |
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Alternative name
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p130 (Rb2)
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Immunogen
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BALB/C mice were injected with a synthetic peptide from p130 corresponding to amino acid 878
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Format
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Ig-fraction
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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130P215
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Specificity
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This antibody reacts with a 130 kD protein. Two retinoblastoma
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Publications
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Howard, Claudio, Gallia et al.: "Retinoblastoma-related protein pRb2/p130 and suppression of tumor growth in vivo." in: Journal of the National Cancer Institute, Vol. 90, Issue 19, pp. 1451-60, 1998 (PubMed).
Baldi, Esposito, De Luca et al.: "Differential expression of the retinoblastoma gene family members pRb/p105, p107, and pRb2/p130 in lung cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 2, Issue 7, pp. 1239-45, 1999 (PubMed).
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Alternatives for antigen "Retinoblastoma-Like 2 (p130) (RBL2)", type "Antibodies"
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Hosts
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Rabbit (8), Mouse (6)
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Reactivities
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Human (14), Rat (Rattus) (7), Mouse (Murine) (5), Chimpanzee (2), Cow (Bovine) (2), Dog (Canine) (2), Horse (Equine) (2)
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Applications
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Western Blotting (WB) (13), Enzyme Immunoassay (EIA) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Immunofluorescence (IF) (3), Immunoprecipitation (IP) (3), Flow Cytometry (FACS) (1), Immunohistochemistry (IHC) (1)
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Epitopes
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Center (1), N-Term (1)
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