ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1) antibody

Antigen: ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1)

Synonyms: CLCS, MDR1, P-GP, PGY1, ABC20, CD243, GP170, MGC163296, Abcb1, Mdr1a, ABCB1, ABCB4, CMDR1, xemdr, p-gp

Clonality: Monoclonal (C494)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN105306

Additional Information

Alternative name: p170 / p-Glycoprotein / MDR

Immunogen: BALB/C mice were injected with SDS-solublized plasma membranes of multidrug-resistant Chinese hamster ovary (CHRB30) and human (CEM/VLB500).

Format: Supernatant

Isotype: IgG2a kappa  (Matching secondary antibodies)

Clone: C494

Specificity: This antibody reacts with a 170 kD protein. Multi-drug resistance (MDR) associated with p-glycoprotein (p170) causes resistance to various drugs in a number of cancer patients. It exists as soluble and membrane associated form. Soluble p-glycoprotein is detected in extracellular fluids of cancer patients, such as malignant ascites and serum.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Adrenal. Cellular localization: Cell membrane

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human adrenal stained with p170/MDR antibody

Publications

Publications: Bani, Brandi, Axiotis et al.: "Detection of P-glycoprotein on endothelial and endocrine cells of the human pancreatic islets by C 494 monoclonal antibody." in: Histochemistry, Vol. 98, Issue 4, pp. 207-9, 1993 (PubMed).

Georges, Bradley, Gariepy et al.: "Detection of P-glycoprotein isoforms by gene-specific monoclonal antibodies." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 1, pp. 152-6, 1990 (PubMed).

Rao, Anthony, Piwnica-Worms: "MDR1 gene-specific monoclonal antibody C494 cross-reacts with pyruvate carboxylase." in: Cancer research, Vol. 54, Issue 6, pp. 1536-41, 1994 (PubMed).