Placental Alkaline Phosphatase (PLAP) antibody

Antigen: Placental Alkaline Phosphatase (PLAP)

Synonyms: DOA1, PLAP, PLA2P, FLJ11281, FLJ12699, cb623, zgc:56568, zgc:66193, wu:fc45d08, wu:fi33e06, PLAA

Clonality: Monoclonal (8B6)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN105666

Additional Information

Immunogen: BALB/C mice were injected with whole cells (Hep-2) expressing placental alkaline phosphatase.

Format: Ascites (prediluted)

Isotype: IgG2a kappa  (Matching secondary antibodies)

Clone: 8B6

Specificity: This antibody is specific to PLAP. This antibody stains germ cells and trophoblastic neoplasms, e.g. seminoma/dysgerminoma, embryonal carcinoma, yolk-sac tumor, choriocarcinoma and carcinoma in-situ of testis.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. Positive Control: Placenta. Cellular localization: Cell membrane

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Enzymes

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Durbin, Milligan, Mather et al.: "Monoclonal antibodies to placental alkaline phosphatase: preclinical evaluation in a human xenograft tumour model of F(ab')2 and Fab fragments." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 2, pp. 59-66, 1988 (PubMed).