Progesterone Receptor (PGR) antibody

Antigen: Progesterone Receptor (PGR)

Synonyms: PR, NR3C3, PR-A, PR-B, BB114106, 9930019P03Rik, ENSMUSG00000074510, PGR, pgr-A, XPR-1, LOC443444, pg, pr, gb:dq017620

Clonality: Monoclonal (HPRa2-hPRa3)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN105891

Additional Information

Alternative name: Progesterone Receptor

Immunogen: BALB/C mice were injected with progesterone receptor from human endometrial carcinoma (EnCa 101) grown in athymic mice.

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: HPRa2-hPRa3

Specificity: This antibody is specific to two proteins 116 kD (triplet) and 81 kD (singlet) known as hormone binding high (B) and low (A) molecular weight forms of human progesterone receptor.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Hormones, Receptors

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human breast carcinoma stained with Progesterone Receptor antibody